Caffeine induced free calcium changes in intact fast- and slow-twitch muscles

L. Ligeti, S. Batkai, T. Ivanics, A. Tóth, D. W. Slaaf, R. S. Reneman

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The sarcoplasmic calcium levels of fast-twitch extensor digitorum longus (EDL) and slow-twitch soleus (SOL) muscle of the rat were measured during caffeine superfusion, to test the hypothesis that the different sensitivity of the two muscle types for this substance is due to different characteristics of the calcium metabolism of the two fiber types. To this end [Ca2+]i was determined in these fiber types. Understanding of [Ca2+]i signal in skeletal muscle is hampered by the lack of [Ca2+]i quantification in the fibers in situ. In the present study, the Indo-1 ratiometric method, using intravital microscopy with DCCD camera for collecting fluorescence images, was adapted to measure [Ca2+]i in vivo. To demonstrate the usefulness of the model we report the first determinations of caffeine induced [Ca2+]i transients in muscle fibers (SOL and EDL). Caffeine - superfused for 2 min. at 37°C: 1, 2, 5, 10 mmol/1 - induced an increase in [Ca2+]i increase in a dose-dependent manner (SOL: 0%, 13.5%, 18.3%, 44.0%; EDL: 0%, 0%, 4.8%, 14.8%, respectively), revealing differences between fibers in threshold concentration and peak responses. These results thus support the hypothesis, that slow-twitch fibers are more sensitive to caffeine than fast-twitch fibers. The different kinetics of the release channels in the two fiber types and the slowed reuptake by the SR may explain the apparent differences in [Ca2+], changes evoked by caffeine.

Original languageEnglish
JournalFASEB Journal
Volume12
Issue number5
Publication statusPublished - Mar 20 1998

Fingerprint

caffeine
Caffeine
Muscle
Calcium
calcium
Muscles
muscles
Fibers
Skeletal Muscle
Dicyclohexylcarbodiimide
Fluorescence
Metabolism
Rats
muscle fibers
cameras
skeletal muscle
Cameras
microscopy
fluorescence
Kinetics

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Ligeti, L., Batkai, S., Ivanics, T., Tóth, A., Slaaf, D. W., & Reneman, R. S. (1998). Caffeine induced free calcium changes in intact fast- and slow-twitch muscles. FASEB Journal, 12(5).

Caffeine induced free calcium changes in intact fast- and slow-twitch muscles. / Ligeti, L.; Batkai, S.; Ivanics, T.; Tóth, A.; Slaaf, D. W.; Reneman, R. S.

In: FASEB Journal, Vol. 12, No. 5, 20.03.1998.

Research output: Contribution to journalArticle

Ligeti, L, Batkai, S, Ivanics, T, Tóth, A, Slaaf, DW & Reneman, RS 1998, 'Caffeine induced free calcium changes in intact fast- and slow-twitch muscles', FASEB Journal, vol. 12, no. 5.
Ligeti L, Batkai S, Ivanics T, Tóth A, Slaaf DW, Reneman RS. Caffeine induced free calcium changes in intact fast- and slow-twitch muscles. FASEB Journal. 1998 Mar 20;12(5).
Ligeti, L. ; Batkai, S. ; Ivanics, T. ; Tóth, A. ; Slaaf, D. W. ; Reneman, R. S. / Caffeine induced free calcium changes in intact fast- and slow-twitch muscles. In: FASEB Journal. 1998 ; Vol. 12, No. 5.
@article{29da3c2573714b1f8d22d3f521f5a6e7,
title = "Caffeine induced free calcium changes in intact fast- and slow-twitch muscles",
abstract = "The sarcoplasmic calcium levels of fast-twitch extensor digitorum longus (EDL) and slow-twitch soleus (SOL) muscle of the rat were measured during caffeine superfusion, to test the hypothesis that the different sensitivity of the two muscle types for this substance is due to different characteristics of the calcium metabolism of the two fiber types. To this end [Ca2+]i was determined in these fiber types. Understanding of [Ca2+]i signal in skeletal muscle is hampered by the lack of [Ca2+]i quantification in the fibers in situ. In the present study, the Indo-1 ratiometric method, using intravital microscopy with DCCD camera for collecting fluorescence images, was adapted to measure [Ca2+]i in vivo. To demonstrate the usefulness of the model we report the first determinations of caffeine induced [Ca2+]i transients in muscle fibers (SOL and EDL). Caffeine - superfused for 2 min. at 37°C: 1, 2, 5, 10 mmol/1 - induced an increase in [Ca2+]i increase in a dose-dependent manner (SOL: 0{\%}, 13.5{\%}, 18.3{\%}, 44.0{\%}; EDL: 0{\%}, 0{\%}, 4.8{\%}, 14.8{\%}, respectively), revealing differences between fibers in threshold concentration and peak responses. These results thus support the hypothesis, that slow-twitch fibers are more sensitive to caffeine than fast-twitch fibers. The different kinetics of the release channels in the two fiber types and the slowed reuptake by the SR may explain the apparent differences in [Ca2+], changes evoked by caffeine.",
author = "L. Ligeti and S. Batkai and T. Ivanics and A. T{\'o}th and Slaaf, {D. W.} and Reneman, {R. S.}",
year = "1998",
month = "3",
day = "20",
language = "English",
volume = "12",
journal = "FASEB Journal",
issn = "0892-6638",
publisher = "FASEB",
number = "5",

}

TY - JOUR

T1 - Caffeine induced free calcium changes in intact fast- and slow-twitch muscles

AU - Ligeti, L.

AU - Batkai, S.

AU - Ivanics, T.

AU - Tóth, A.

AU - Slaaf, D. W.

AU - Reneman, R. S.

PY - 1998/3/20

Y1 - 1998/3/20

N2 - The sarcoplasmic calcium levels of fast-twitch extensor digitorum longus (EDL) and slow-twitch soleus (SOL) muscle of the rat were measured during caffeine superfusion, to test the hypothesis that the different sensitivity of the two muscle types for this substance is due to different characteristics of the calcium metabolism of the two fiber types. To this end [Ca2+]i was determined in these fiber types. Understanding of [Ca2+]i signal in skeletal muscle is hampered by the lack of [Ca2+]i quantification in the fibers in situ. In the present study, the Indo-1 ratiometric method, using intravital microscopy with DCCD camera for collecting fluorescence images, was adapted to measure [Ca2+]i in vivo. To demonstrate the usefulness of the model we report the first determinations of caffeine induced [Ca2+]i transients in muscle fibers (SOL and EDL). Caffeine - superfused for 2 min. at 37°C: 1, 2, 5, 10 mmol/1 - induced an increase in [Ca2+]i increase in a dose-dependent manner (SOL: 0%, 13.5%, 18.3%, 44.0%; EDL: 0%, 0%, 4.8%, 14.8%, respectively), revealing differences between fibers in threshold concentration and peak responses. These results thus support the hypothesis, that slow-twitch fibers are more sensitive to caffeine than fast-twitch fibers. The different kinetics of the release channels in the two fiber types and the slowed reuptake by the SR may explain the apparent differences in [Ca2+], changes evoked by caffeine.

AB - The sarcoplasmic calcium levels of fast-twitch extensor digitorum longus (EDL) and slow-twitch soleus (SOL) muscle of the rat were measured during caffeine superfusion, to test the hypothesis that the different sensitivity of the two muscle types for this substance is due to different characteristics of the calcium metabolism of the two fiber types. To this end [Ca2+]i was determined in these fiber types. Understanding of [Ca2+]i signal in skeletal muscle is hampered by the lack of [Ca2+]i quantification in the fibers in situ. In the present study, the Indo-1 ratiometric method, using intravital microscopy with DCCD camera for collecting fluorescence images, was adapted to measure [Ca2+]i in vivo. To demonstrate the usefulness of the model we report the first determinations of caffeine induced [Ca2+]i transients in muscle fibers (SOL and EDL). Caffeine - superfused for 2 min. at 37°C: 1, 2, 5, 10 mmol/1 - induced an increase in [Ca2+]i increase in a dose-dependent manner (SOL: 0%, 13.5%, 18.3%, 44.0%; EDL: 0%, 0%, 4.8%, 14.8%, respectively), revealing differences between fibers in threshold concentration and peak responses. These results thus support the hypothesis, that slow-twitch fibers are more sensitive to caffeine than fast-twitch fibers. The different kinetics of the release channels in the two fiber types and the slowed reuptake by the SR may explain the apparent differences in [Ca2+], changes evoked by caffeine.

UR - http://www.scopus.com/inward/record.url?scp=29344469989&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=29344469989&partnerID=8YFLogxK

M3 - Article

VL - 12

JO - FASEB Journal

JF - FASEB Journal

SN - 0892-6638

IS - 5

ER -