Caenimicrobium hargitense gen. nov., sp. nov., a new member of the family Alcaligenaceae (Betaproteobacteria) isolated from activated sludge

Tamás Felföldi, Peter Schumann, Anikó Mentes, Zsuzsa Kéki, István Máthé, E. Tóth

Research output: Contribution to journalComment/debate

2 Citations (Scopus)

Abstract

A new betaproteobacterium, CGII-59m2T, was isolated from an activated sludge bioreactor which treated landfill leachate. The 16S rRNA gene sequence analysis revealed that strain CGII-59m2T belonged to the family Alcaligenaceae and shared the highest pairwise similarity values with Parapusillimonas granuli LMG 24012T (97.7 %), various species of the genus Bordetella (97.3-97.0 %) and Candidimonas nitroreducens LMG 24812T (97.0 %). Cells of strain CGII-59m2T were rod-shaped, non-motile, and oxidase- and catalase-positive. The predominant fatty acids were C16: 1ω7c, C16: 0, cyclo C17: 0 and C18: 1ω7c, the major respiratory quinone was Q-8, and the main polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and an unknown phospholipid. The G+C content of the genomic DNA of strain CGII-59m2T was 62.3 mol%. The new bacterium can be distinguished from the closely related type strains based on its non-motile cells and its high C16: 1ω7c fatty acid content. On the basis of the phenotypic, chemotaxonomic and molecular data, strain CGII-59m2T is considered to represent a novel species of a new genus, for which the name Caenimicrobium hargitense gen. nov., sp. nov. is proposed. The type strain is CGII-59m2T (=DSM 29806T=NCAIM B.02615T).

Original languageEnglish
Article number001674
Pages (from-to)627-632
Number of pages6
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume67
Issue number3
DOIs
Publication statusPublished - Apr 1 2017

Fingerprint

Alcaligenaceae
Betaproteobacteria
beta-Proteobacteria
activated sludge
Sewage
Fatty Acids
fatty acid
Chemical Water Pollutants
Bordetella
Phosphatidylglycerols
Cardiolipins
new species
Base Composition
Bioreactors
phospholipid
rRNA Genes
bioreactor
Catalase
Names
new genus

Keywords

  • Activated sludge
  • Caenimicrobium
  • Class betaproteobacteria
  • Family alcaligenaceae
  • New genus

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Caenimicrobium hargitense gen. nov., sp. nov., a new member of the family Alcaligenaceae (Betaproteobacteria) isolated from activated sludge. / Felföldi, Tamás; Schumann, Peter; Mentes, Anikó; Kéki, Zsuzsa; Máthé, István; Tóth, E.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 67, No. 3, 001674, 01.04.2017, p. 627-632.

Research output: Contribution to journalComment/debate

@article{1dec5e3c883b463d8a404b659bca5c82,
title = "Caenimicrobium hargitense gen. nov., sp. nov., a new member of the family Alcaligenaceae (Betaproteobacteria) isolated from activated sludge",
abstract = "A new betaproteobacterium, CGII-59m2T, was isolated from an activated sludge bioreactor which treated landfill leachate. The 16S rRNA gene sequence analysis revealed that strain CGII-59m2T belonged to the family Alcaligenaceae and shared the highest pairwise similarity values with Parapusillimonas granuli LMG 24012T (97.7 {\%}), various species of the genus Bordetella (97.3-97.0 {\%}) and Candidimonas nitroreducens LMG 24812T (97.0 {\%}). Cells of strain CGII-59m2T were rod-shaped, non-motile, and oxidase- and catalase-positive. The predominant fatty acids were C16: 1ω7c, C16: 0, cyclo C17: 0 and C18: 1ω7c, the major respiratory quinone was Q-8, and the main polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and an unknown phospholipid. The G+C content of the genomic DNA of strain CGII-59m2T was 62.3 mol{\%}. The new bacterium can be distinguished from the closely related type strains based on its non-motile cells and its high C16: 1ω7c fatty acid content. On the basis of the phenotypic, chemotaxonomic and molecular data, strain CGII-59m2T is considered to represent a novel species of a new genus, for which the name Caenimicrobium hargitense gen. nov., sp. nov. is proposed. The type strain is CGII-59m2T (=DSM 29806T=NCAIM B.02615T).",
keywords = "Activated sludge, Caenimicrobium, Class betaproteobacteria, Family alcaligenaceae, New genus",
author = "Tam{\'a}s Felf{\"o}ldi and Peter Schumann and Anik{\'o} Mentes and Zsuzsa K{\'e}ki and Istv{\'a}n M{\'a}th{\'e} and E. T{\'o}th",
year = "2017",
month = "4",
day = "1",
doi = "10.1099/ijsem.0.001674",
language = "English",
volume = "67",
pages = "627--632",
journal = "International Journal of Systematic and Evolutionary Microbiology",
issn = "1466-5026",
publisher = "Society for General Microbiology",
number = "3",

}

TY - JOUR

T1 - Caenimicrobium hargitense gen. nov., sp. nov., a new member of the family Alcaligenaceae (Betaproteobacteria) isolated from activated sludge

AU - Felföldi, Tamás

AU - Schumann, Peter

AU - Mentes, Anikó

AU - Kéki, Zsuzsa

AU - Máthé, István

AU - Tóth, E.

PY - 2017/4/1

Y1 - 2017/4/1

N2 - A new betaproteobacterium, CGII-59m2T, was isolated from an activated sludge bioreactor which treated landfill leachate. The 16S rRNA gene sequence analysis revealed that strain CGII-59m2T belonged to the family Alcaligenaceae and shared the highest pairwise similarity values with Parapusillimonas granuli LMG 24012T (97.7 %), various species of the genus Bordetella (97.3-97.0 %) and Candidimonas nitroreducens LMG 24812T (97.0 %). Cells of strain CGII-59m2T were rod-shaped, non-motile, and oxidase- and catalase-positive. The predominant fatty acids were C16: 1ω7c, C16: 0, cyclo C17: 0 and C18: 1ω7c, the major respiratory quinone was Q-8, and the main polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and an unknown phospholipid. The G+C content of the genomic DNA of strain CGII-59m2T was 62.3 mol%. The new bacterium can be distinguished from the closely related type strains based on its non-motile cells and its high C16: 1ω7c fatty acid content. On the basis of the phenotypic, chemotaxonomic and molecular data, strain CGII-59m2T is considered to represent a novel species of a new genus, for which the name Caenimicrobium hargitense gen. nov., sp. nov. is proposed. The type strain is CGII-59m2T (=DSM 29806T=NCAIM B.02615T).

AB - A new betaproteobacterium, CGII-59m2T, was isolated from an activated sludge bioreactor which treated landfill leachate. The 16S rRNA gene sequence analysis revealed that strain CGII-59m2T belonged to the family Alcaligenaceae and shared the highest pairwise similarity values with Parapusillimonas granuli LMG 24012T (97.7 %), various species of the genus Bordetella (97.3-97.0 %) and Candidimonas nitroreducens LMG 24812T (97.0 %). Cells of strain CGII-59m2T were rod-shaped, non-motile, and oxidase- and catalase-positive. The predominant fatty acids were C16: 1ω7c, C16: 0, cyclo C17: 0 and C18: 1ω7c, the major respiratory quinone was Q-8, and the main polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and an unknown phospholipid. The G+C content of the genomic DNA of strain CGII-59m2T was 62.3 mol%. The new bacterium can be distinguished from the closely related type strains based on its non-motile cells and its high C16: 1ω7c fatty acid content. On the basis of the phenotypic, chemotaxonomic and molecular data, strain CGII-59m2T is considered to represent a novel species of a new genus, for which the name Caenimicrobium hargitense gen. nov., sp. nov. is proposed. The type strain is CGII-59m2T (=DSM 29806T=NCAIM B.02615T).

KW - Activated sludge

KW - Caenimicrobium

KW - Class betaproteobacteria

KW - Family alcaligenaceae

KW - New genus

UR - http://www.scopus.com/inward/record.url?scp=85016955369&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85016955369&partnerID=8YFLogxK

U2 - 10.1099/ijsem.0.001674

DO - 10.1099/ijsem.0.001674

M3 - Comment/debate

C2 - 27902326

AN - SCOPUS:85016955369

VL - 67

SP - 627

EP - 632

JO - International Journal of Systematic and Evolutionary Microbiology

JF - International Journal of Systematic and Evolutionary Microbiology

SN - 1466-5026

IS - 3

M1 - 001674

ER -