C5a receptor expression by TGW neuroblastoma cells

Imre Farkas, Lajos Baranyi, Yoko Kaneko, Z. Liposits, Takayuki Yamamoto, Hidechika Okada

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

We recently reported that not only lymphoid cells, but cells of neuronal origin may harbor C5a receptors (C5aR) as suggested by results of RT-PCR testing and that an apoptotic pathway is associated with the C5aR. To determine whether C5aR is expressed as an integral membrane protein, we generated mono- and polyclonal anti-CSaR antibodies. Flow cytometry showed a low-level expression of C5aR in TGW neuroblastoma cells. Epitope mapping suggested that a conformation change in C5aR occurs when exposed to C5a. Although an aphysiologically high concentration of C5a is necessary for inducing a transient increase in the intracellular Ca2+ level, TGW cells do employ the signal transduction pathway associated with C5aR, suggesting that these cells may serve as putative model for C5aR-expressing neurons.

Original languageEnglish
Pages (from-to)3021-3025
Number of pages5
JournalNeuroReport
Volume10
Issue number14
Publication statusPublished - Sep 29 1999

Fingerprint

Anaphylatoxin C5a Receptor
Neuroblastoma
Epitope Mapping
Anti-Idiotypic Antibodies
Signal Transduction
Flow Cytometry
Membrane Proteins
Lymphocytes
Neurons
Polymerase Chain Reaction

Keywords

  • Alzheimer's disease
  • Antisense homology box
  • C5a
  • C5a receptor
  • Complementary peptide
  • Neuroblastoma
  • Neuron

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Farkas, I., Baranyi, L., Kaneko, Y., Liposits, Z., Yamamoto, T., & Okada, H. (1999). C5a receptor expression by TGW neuroblastoma cells. NeuroReport, 10(14), 3021-3025.

C5a receptor expression by TGW neuroblastoma cells. / Farkas, Imre; Baranyi, Lajos; Kaneko, Yoko; Liposits, Z.; Yamamoto, Takayuki; Okada, Hidechika.

In: NeuroReport, Vol. 10, No. 14, 29.09.1999, p. 3021-3025.

Research output: Contribution to journalArticle

Farkas, I, Baranyi, L, Kaneko, Y, Liposits, Z, Yamamoto, T & Okada, H 1999, 'C5a receptor expression by TGW neuroblastoma cells', NeuroReport, vol. 10, no. 14, pp. 3021-3025.
Farkas I, Baranyi L, Kaneko Y, Liposits Z, Yamamoto T, Okada H. C5a receptor expression by TGW neuroblastoma cells. NeuroReport. 1999 Sep 29;10(14):3021-3025.
Farkas, Imre ; Baranyi, Lajos ; Kaneko, Yoko ; Liposits, Z. ; Yamamoto, Takayuki ; Okada, Hidechika. / C5a receptor expression by TGW neuroblastoma cells. In: NeuroReport. 1999 ; Vol. 10, No. 14. pp. 3021-3025.
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