C5a receptor expression by TGW neuroblastoma cells

Imre Farkas, Lajos Baranyi, Yoko Kaneko, Zsolt Liposits, Takayuki Yamamoto, Hidechika Okada

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

We recently reported that not only lymphoid cells, but cells of neuronal origin may harbor C5a receptors (C5aR) as suggested by results of RT-PCR testing and that an apoptotic pathway is associated with the C5aR. To determine whether C5aR is expressed as an integral membrane protein, we generated mono- and polyclonal anti-CSaR antibodies. Flow cytometry showed a low-level expression of C5aR in TGW neuroblastoma cells. Epitope mapping suggested that a conformation change in C5aR occurs when exposed to C5a. Although an aphysiologically high concentration of C5a is necessary for inducing a transient increase in the intracellular Ca2+ level, TGW cells do employ the signal transduction pathway associated with C5aR, suggesting that these cells may serve as putative model for C5aR-expressing neurons.

Original languageEnglish
Pages (from-to)3021-3025
Number of pages5
JournalNeuroreport
Volume10
Issue number14
DOIs
Publication statusPublished - Sep 29 1999

Keywords

  • Alzheimer's disease
  • Antisense homology box
  • C5a
  • C5a receptor
  • Complementary peptide
  • Neuroblastoma
  • Neuron

ASJC Scopus subject areas

  • Neuroscience(all)

Fingerprint Dive into the research topics of 'C5a receptor expression by TGW neuroblastoma cells'. Together they form a unique fingerprint.

  • Cite this

    Farkas, I., Baranyi, L., Kaneko, Y., Liposits, Z., Yamamoto, T., & Okada, H. (1999). C5a receptor expression by TGW neuroblastoma cells. Neuroreport, 10(14), 3021-3025. https://doi.org/10.1097/00001756-199909290-00027