C-terminal peptides of interleukin-6 modulate the expression of junB protooncogene and the production of fibrinogen by HepG2 cells

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5 Citations (Scopus)

Abstract

Interleukin-6 (IL-6) is a 185 amino acid residue helical cytokine with various biological activities (e.g. B cell development, acute phase reaction). We have investigated the role of the 168-185 C-terminal region of IL-6 in the induction of fibrinogen synthesis and expression of junB mRNA using synthetic peptides corresponding to this region. Circular dichroism spectroscopy data suggest that even truncated peptides have a strong tendency to adopt an ordered conformation. Peptides were tested alone or in combination with recombinant hIL-6 on an IL-6 responsive human hepatoma HepG2 cell line. The expression of the protooncogene junB monitored by competitive RT-PCR represents an early, while the fibrinogen production detected by sandwich ELISA a late, marker of IL-6 initiated events. We found that peptides - depending on their structure - modulate spontaneous as well as IL-6 induced fibrinogen production and/or mRNA expression of junB by exhibiting inhibition (in the presence of IL-6) or stimulation (in the absence of IL-6).

Original languageEnglish
Pages (from-to)669-676
Number of pages8
JournalBiological Chemistry
Volume382
Issue number4
DOIs
Publication statusPublished - Apr 1 2001

Keywords

  • Acute phase protein
  • Circular dichroism spectroscopy
  • HepG2
  • IL-6 related synthetic peptides

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Clinical Biochemistry

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