Buffer and pH dependence of the retention of phenylthiocarbamylamino acids in reversed-phase high-performance liquid chromatography

Magdolna Morvai, Valéria Fábián, Ibolya Molnár-Perk

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

An exhaustive study was made to find the optimum conditions for the determination of phenylthiocarbamyl (PTC)-amino acids. The effects of the type of salt dissolved in the eluent (sodium or ammonium acetate), the pH of the eluent, the presence of additives such as triethylamine and phosphate and the maximum storage time of the dissolved derivatives were investigated. On Hypersil or·Nucleosil columns (15 cm x 4.6 mm I.D.; 5 μm) the determination of 21 PTC-amino acids [including cyst(e)ine, tryptophan and ornithine] require 22 min. The reproducibility of the measurements was < 5.0% (relative standard deviation).

Original languageEnglish
Pages (from-to)87-92
Number of pages6
JournalJournal of Chromatography A
Volume600
Issue number1
DOIs
Publication statusPublished - May 27 1992

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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