Broncho-Vaxom® inhibits histamine release from rat mast cells induced by compound 48/80 and ionophore A23187

A. Németh, P. Magyar

Research output: Contribution to journalArticle

Abstract

Broncho-Vaxom (BV) inhibited in dose-dependent manner the release of histamine from and degranulation of isolated rat peritoneal mast cells stimulated with compound 48/80 and the ionophore A23187. Inhibition persisted after removal of BV from the incubation medium before stimulation, but did not occur when bovine serum albumin (BSA) was used instead of BV. Binding of BV to mast cells was observed by electron microscopy on cells that had been incubated with colloidal-gold labelled BV. There was no significant difference between the binding of BV gold and BSA gold to the mast cells. Washing before fixation removed most of the BV gold from the cells. This study establishes BV as an in vitro histamine release inhibitor.

Original languageEnglish
Pages (from-to)43-45
Number of pages3
JournalAgents and Actions
Volume27
Issue number1-2
DOIs
Publication statusPublished - Apr 1989

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p-Methoxy-N-methylphenethylamine
Histamine Release
Ionophores
Calcimycin
Mast Cells
Histamine
Rats
Gold
Bovine Serum Albumin
Gold Colloid
Broncho-Vaxom
Washing
Electron microscopy
Electron Microscopy

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology (medical)

Cite this

Broncho-Vaxom® inhibits histamine release from rat mast cells induced by compound 48/80 and ionophore A23187. / Németh, A.; Magyar, P.

In: Agents and Actions, Vol. 27, No. 1-2, 04.1989, p. 43-45.

Research output: Contribution to journalArticle

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