Breed-Specific Detection of Mangalica Meat in Food Products

R. Szántó-Egész, A. Jánosi, A. Mohr, G. Szalai, E. Koppányné Szabó, A. Micsinai, R. Sipos, J. Rátky, I. Anton, A. Zsolnai

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A fast and reliable diagnostic system has been developed for the detection of Mangalica meat in foods. This qualitative test is based on a recombinase polymerase amplification which can be performed on the field, in situ, where it may be necessary to determine Mangalica content in food products at once. The required equipments for the procedure are pipettes, a portable homogenizer and a portable thermostat. DNA amplification is carried out at a constant temperature, and the detection is based on antibody reaction. The detection limit is one copy of the target sequence in 1 μl reaction volume. The test can be used for uncovering falsification of local brands on the spot within a very short (25–45 min) period of time. The present approach can be adopted for the detection of other food ingredients, if the species-specific target DNA sequence is known, e.g. in case of chicken, turkey, horse, and cattle.

Original languageEnglish
Pages (from-to)889-894
Number of pages6
JournalFood Analytical Methods
Volume9
Issue number4
DOIs
Publication statusPublished - Apr 1 2016

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Keywords

  • Breed identification
  • DNA
  • Food composition
  • Pork
  • Recombinase polymerase amplification

ASJC Scopus subject areas

  • Analytical Chemistry
  • Food Science
  • Applied Microbiology and Biotechnology
  • Safety, Risk, Reliability and Quality
  • Safety Research

Cite this

Szántó-Egész, R., Jánosi, A., Mohr, A., Szalai, G., Szabó, E. K., Micsinai, A., Sipos, R., Rátky, J., Anton, I., & Zsolnai, A. (2016). Breed-Specific Detection of Mangalica Meat in Food Products. Food Analytical Methods, 9(4), 889-894. https://doi.org/10.1007/s12161-015-0261-0