Brassinosteroids rescue the deficiency of CYP90, a cytochrome P450, controlling cell elongation and de-etiolation in Arabidopsis

Miklós Szekeres, Kinga Németh, Zsuzsanna Koncz-Kálmán, Jaideep Mathur, Annette Kauschmann, Thomas Altmann, George P. Rédei, Ferenc Nagy, Jeff Schell, Csaba Koncz

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Abstract

The cpd mutation localized by T-DNA tagging on Arabidopsis chromosome 5- 14.3 inhibits cell elongation controlled by the ecdysone-like brassinosteroid hormone brassinolide. The cpd mutant displays de-etiolation and derepression of light-induced genes in the dark, as well as dwarfism, male sterility, and activation of stress-regulated genes in the light. The CPD gene encodes a cytochrome P450 (CYP90) sharing homologous domains with steroid hydroxylases. The phenotype of the cpd mutant is restored to wild type both by feeding with C23-hydroxylated brassinolide precursors and by ectopic overexpression of the CPD cDNA. Brassinosteroids also compensate for different cell elongation defects of Arabidopsis der, cop, fus, and axr2 mutants, indicating that these steroids play an essential role in the regulation of plant development.

Original languageEnglish
Pages (from-to)171-182
Number of pages12
JournalCell
Volume85
Issue number2
DOIs
Publication statusPublished - Apr 19 1996

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ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Szekeres, M., Németh, K., Koncz-Kálmán, Z., Mathur, J., Kauschmann, A., Altmann, T., Rédei, G. P., Nagy, F., Schell, J., & Koncz, C. (1996). Brassinosteroids rescue the deficiency of CYP90, a cytochrome P450, controlling cell elongation and de-etiolation in Arabidopsis. Cell, 85(2), 171-182. https://doi.org/10.1016/S0092-8674(00)81094-6