Brain-derived neurotrophic factor restores long-term potentiation in polysialic acid-neural cell adhesion molecule-deficient hippocampus

D. Muller, Z. Djebbara-Hannas, P. Jourdain, L. Vutskits, P. Durbec, G. Rougon, J. Kiss

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Abstract

The neural cell adhesion molecule (NCAM) and its polysialylated form (PSA-NCAM) contribute to long-term potentiation (LTP) in the CA1 hippocampus. Here we report that the deficient LTP found in slices prepared from NCAM knockout mice and in organotypic slice cultures treated with Endo-N, an enzyme that cleaves the PSA moiety of NCAM, can be rescued by brain-derived neurotrophic factor (BDNF). This effect is not reproduced by nerve growth factor, but can be obtained with high concentrations of NT4/5. The effect of BDNF cannot be accounted for by modifications of N-methyl-D-aspartate receptor-dependent responses or of high-frequency bursts. PSA-NCAM, however, could directly interact with BDNF. Exogenous application of PSA residues or recombinant PSA-NCAM also prevents LTP. Furthermore trkB phosphorylation, and thus BDNF signaling, is reduced in both NCAM knockout mice and Endo-N-treated slice cultures. These results suggest that one action of PSA-NCAM could be to sensitize pyramidal neurons to BDNF, thereby modulating activity-dependent synaptic plasticity.

Original languageEnglish
Pages (from-to)4315-4320
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume97
Issue number8
DOIs
Publication statusPublished - Apr 11 2000

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Neural Cell Adhesion Molecules
Long-Term Potentiation
Brain-Derived Neurotrophic Factor
Hippocampus
Knockout Mice
Neuronal Plasticity
polysialic acid
Pyramidal Cells
Nerve Growth Factor
N-Methyl-D-Aspartate Receptors
Phosphorylation
Enzymes

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Brain-derived neurotrophic factor restores long-term potentiation in polysialic acid-neural cell adhesion molecule-deficient hippocampus. / Muller, D.; Djebbara-Hannas, Z.; Jourdain, P.; Vutskits, L.; Durbec, P.; Rougon, G.; Kiss, J.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, No. 8, 11.04.2000, p. 4315-4320.

Research output: Contribution to journalArticle

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