Bradyrhizobium BclA Is a Peptide Transporter Required for Bacterial Differentiation in Symbiosis with Aeschynomene Legumes

Ibtissem Guefrachi, Olivier Pierre, Tatiana Timchenko, Benoît Alunni, Quentin Barrière, Pierre Czernic, José Antonio Villaécija-Aguilar, Camille Verly, Mickaël Bourge, Joël Fardoux, Mohamed Mars, É. Kondorosi, Eric Giraud, Peter Mergaert

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Nodules of legume plants are highly integrated symbiotic systems shaped by millions of years of evolution. They harbor nitrogen-fixing rhizobium bacteria called bacteroids. Several legume species produce peptides called nodule-specific cysteine-rich (NCR) peptides in the symbiotic nodule cells which house the bacteroids. NCR peptides are related to antimicrobial peptides of innate immunity. They induce the endosymbionts into a differentiated, enlarged, and polyploid state. The bacterial symbionts, on their side, evolved functions for the response to the NCR peptides. Here, we identified the bclA gene of Bradyrhizobium sp. strains ORS278 and ORS285, which is required for the formation of differentiated and functional bacteroids in the nodules of the NCR peptide-producing Aeschynomene legumes. The BclA ABC transporter promotes the import of NCR peptides and provides protection against the antimicrobial activity of these peptides. Moreover, BclA can complement the role of the related BacA transporter of Sinorhizobium meliloti, which has a similar symbiotic function in the interaction with Medicago legumes.

Original languageEnglish
Pages (from-to)1155-1166
Number of pages12
JournalMolecular Plant-Microbe Interactions
Volume28
Issue number11
DOIs
Publication statusPublished - Nov 1 2015

Fingerprint

peptide transporters
Aeschynomene
Bradyrhizobium
Symbiosis
Fabaceae
symbiosis
legumes
peptides
cysteine
Peptides
Cysteine
Ensifer meliloti
ABC transporters
Medicago
Sinorhizobium meliloti
antimicrobial peptides
endosymbionts
Rhizobium
Polyploidy
ATP-Binding Cassette Transporters

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science

Cite this

Bradyrhizobium BclA Is a Peptide Transporter Required for Bacterial Differentiation in Symbiosis with Aeschynomene Legumes. / Guefrachi, Ibtissem; Pierre, Olivier; Timchenko, Tatiana; Alunni, Benoît; Barrière, Quentin; Czernic, Pierre; Villaécija-Aguilar, José Antonio; Verly, Camille; Bourge, Mickaël; Fardoux, Joël; Mars, Mohamed; Kondorosi, É.; Giraud, Eric; Mergaert, Peter.

In: Molecular Plant-Microbe Interactions, Vol. 28, No. 11, 01.11.2015, p. 1155-1166.

Research output: Contribution to journalArticle

Guefrachi, I, Pierre, O, Timchenko, T, Alunni, B, Barrière, Q, Czernic, P, Villaécija-Aguilar, JA, Verly, C, Bourge, M, Fardoux, J, Mars, M, Kondorosi, É, Giraud, E & Mergaert, P 2015, 'Bradyrhizobium BclA Is a Peptide Transporter Required for Bacterial Differentiation in Symbiosis with Aeschynomene Legumes', Molecular Plant-Microbe Interactions, vol. 28, no. 11, pp. 1155-1166. https://doi.org/10.1094/MPMI-04-15-0094-R
Guefrachi, Ibtissem ; Pierre, Olivier ; Timchenko, Tatiana ; Alunni, Benoît ; Barrière, Quentin ; Czernic, Pierre ; Villaécija-Aguilar, José Antonio ; Verly, Camille ; Bourge, Mickaël ; Fardoux, Joël ; Mars, Mohamed ; Kondorosi, É. ; Giraud, Eric ; Mergaert, Peter. / Bradyrhizobium BclA Is a Peptide Transporter Required for Bacterial Differentiation in Symbiosis with Aeschynomene Legumes. In: Molecular Plant-Microbe Interactions. 2015 ; Vol. 28, No. 11. pp. 1155-1166.
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