Both interferon (IFN)α and IFNγ inhibit histidine decarboxylase expression in the HT168 human melanoma cell line

E. Heninger, A. Falus, Z. Darvas, C. Szalai, M. Zsinkó, Z. Pós, H. Hegyesi

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Objective: Histamine plays an important role in a series of processes including inflammation, allergy, gastric acid secretion, neurotransmission, embryogenesis and in various tumours. Histidine decarboxylase (HDC), the enzyme solely responsible for generation of histamine is expressed in many cells including regenerating and tumour cells. HDC expression is regulated by multiple tissue factors, e.g. various cytokines and growth hormones. In this study the effect of interferon α and interferon γ on the expression of HDC and on cell proliferation in vitro on melanoma cell line. Methods: We used recombinant human interferon α, interferon γ and human melanoma cell line HT168. Results: Our data show that both IFNα and IFNγ decreased the HDC mRNA and protein expression, though with dissimilar kinetics. IFNγ strongly suppresses the proliferation at 72 h, while IFNα has a more moderate effect. Conclusions: Since previously the inhibitory effect of histamine on gene expression of interferon γ was detected, a reciprocal inhibition between histamine and IFN γ is proposed.

Original languageEnglish
Pages (from-to)393-397
Number of pages5
JournalInflammation Research
Volume49
Issue number8
DOIs
Publication statusPublished - 2000

Fingerprint

Histidine Decarboxylase
Interferons
Melanoma
Cells
Cell Line
Histamine
Tumors
Allergies
Gastric Acid
Cell proliferation
Thromboplastin
Gene expression
Synaptic Transmission
Growth Hormone
Embryonic Development
Neoplasms
Hypersensitivity
Cell Proliferation

Keywords

  • Histidine decarboxylase
  • Interferon
  • Melanoma

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology (medical)
  • Immunology
  • Cell Biology

Cite this

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title = "Both interferon (IFN)α and IFNγ inhibit histidine decarboxylase expression in the HT168 human melanoma cell line",
abstract = "Objective: Histamine plays an important role in a series of processes including inflammation, allergy, gastric acid secretion, neurotransmission, embryogenesis and in various tumours. Histidine decarboxylase (HDC), the enzyme solely responsible for generation of histamine is expressed in many cells including regenerating and tumour cells. HDC expression is regulated by multiple tissue factors, e.g. various cytokines and growth hormones. In this study the effect of interferon α and interferon γ on the expression of HDC and on cell proliferation in vitro on melanoma cell line. Methods: We used recombinant human interferon α, interferon γ and human melanoma cell line HT168. Results: Our data show that both IFNα and IFNγ decreased the HDC mRNA and protein expression, though with dissimilar kinetics. IFNγ strongly suppresses the proliferation at 72 h, while IFNα has a more moderate effect. Conclusions: Since previously the inhibitory effect of histamine on gene expression of interferon γ was detected, a reciprocal inhibition between histamine and IFN γ is proposed.",
keywords = "Histidine decarboxylase, Interferon, Melanoma",
author = "E. Heninger and A. Falus and Z. Darvas and C. Szalai and M. Zsink{\'o} and Z. P{\'o}s and H. Hegyesi",
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TY - JOUR

T1 - Both interferon (IFN)α and IFNγ inhibit histidine decarboxylase expression in the HT168 human melanoma cell line

AU - Heninger, E.

AU - Falus, A.

AU - Darvas, Z.

AU - Szalai, C.

AU - Zsinkó, M.

AU - Pós, Z.

AU - Hegyesi, H.

PY - 2000

Y1 - 2000

N2 - Objective: Histamine plays an important role in a series of processes including inflammation, allergy, gastric acid secretion, neurotransmission, embryogenesis and in various tumours. Histidine decarboxylase (HDC), the enzyme solely responsible for generation of histamine is expressed in many cells including regenerating and tumour cells. HDC expression is regulated by multiple tissue factors, e.g. various cytokines and growth hormones. In this study the effect of interferon α and interferon γ on the expression of HDC and on cell proliferation in vitro on melanoma cell line. Methods: We used recombinant human interferon α, interferon γ and human melanoma cell line HT168. Results: Our data show that both IFNα and IFNγ decreased the HDC mRNA and protein expression, though with dissimilar kinetics. IFNγ strongly suppresses the proliferation at 72 h, while IFNα has a more moderate effect. Conclusions: Since previously the inhibitory effect of histamine on gene expression of interferon γ was detected, a reciprocal inhibition between histamine and IFN γ is proposed.

AB - Objective: Histamine plays an important role in a series of processes including inflammation, allergy, gastric acid secretion, neurotransmission, embryogenesis and in various tumours. Histidine decarboxylase (HDC), the enzyme solely responsible for generation of histamine is expressed in many cells including regenerating and tumour cells. HDC expression is regulated by multiple tissue factors, e.g. various cytokines and growth hormones. In this study the effect of interferon α and interferon γ on the expression of HDC and on cell proliferation in vitro on melanoma cell line. Methods: We used recombinant human interferon α, interferon γ and human melanoma cell line HT168. Results: Our data show that both IFNα and IFNγ decreased the HDC mRNA and protein expression, though with dissimilar kinetics. IFNγ strongly suppresses the proliferation at 72 h, while IFNα has a more moderate effect. Conclusions: Since previously the inhibitory effect of histamine on gene expression of interferon γ was detected, a reciprocal inhibition between histamine and IFN γ is proposed.

KW - Histidine decarboxylase

KW - Interferon

KW - Melanoma

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