Boronic acid-lectin affinity chromatography. 1. Simultaneous glycoprotein binding with selective or combined elution

Alex Monzo, Guenther K. Bonn, András Guttman

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

We introduce a novel combination of boronic acid affinity chromatography with lectin affinity chromatography, dubbed as boronic acid-lectin affinity chromatography (BLAC). Concanavalin A and wheat germ agglutinin lectins were mixed with the pesudo-lectin boronic acid to form the BLAC affinity column and their performance was evaluated with standard glycoproteins. Optimization of the binding and elution buffers for the BLAC system is described. The BLAC columns were employed to isolate glycoproteins of interest using both selective and/or combined elution.

Original languageEnglish
Pages (from-to)2097-2102
Number of pages6
JournalAnalytical and Bioanalytical Chemistry
Volume389
Issue number7-8
DOIs
Publication statusPublished - Dec 1 2007

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Keywords

  • Affinity chromatography
  • Electrophoresis
  • Lectin
  • Pseudo-lectin

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

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