Boronic acid-lectin affinity chromatography. 1. Simultaneous glycoprotein binding with selective or combined elution

Alex Monzo, Guenther K. Bonn, A. Guttman

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

We introduce a novel combination of boronic acid affinity chromatography with lectin affinity chromatography, dubbed as boronic acid-lectin affinity chromatography (BLAC). Concanavalin A and wheat germ agglutinin lectins were mixed with the pesudo-lectin boronic acid to form the BLAC affinity column and their performance was evaluated with standard glycoproteins. Optimization of the binding and elution buffers for the BLAC system is described. The BLAC columns were employed to isolate glycoproteins of interest using both selective and/or combined elution.

Original languageEnglish
Pages (from-to)2097-2102
Number of pages6
JournalAnalytical and Bioanalytical Chemistry
Volume389
Issue number7-8
DOIs
Publication statusPublished - Dec 2007

Fingerprint

Boronic Acids
Affinity chromatography
Affinity Chromatography
Lectins
Glycoproteins
Wheat Germ Agglutinins
Concanavalin A
Buffers

Keywords

  • Affinity chromatography
  • Electrophoresis
  • Lectin
  • Pseudo-lectin

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry

Cite this

Boronic acid-lectin affinity chromatography. 1. Simultaneous glycoprotein binding with selective or combined elution. / Monzo, Alex; Bonn, Guenther K.; Guttman, A.

In: Analytical and Bioanalytical Chemistry, Vol. 389, No. 7-8, 12.2007, p. 2097-2102.

Research output: Contribution to journalArticle

@article{cddff076e94d4518acf50b73df2dc961,
title = "Boronic acid-lectin affinity chromatography. 1. Simultaneous glycoprotein binding with selective or combined elution",
abstract = "We introduce a novel combination of boronic acid affinity chromatography with lectin affinity chromatography, dubbed as boronic acid-lectin affinity chromatography (BLAC). Concanavalin A and wheat germ agglutinin lectins were mixed with the pesudo-lectin boronic acid to form the BLAC affinity column and their performance was evaluated with standard glycoproteins. Optimization of the binding and elution buffers for the BLAC system is described. The BLAC columns were employed to isolate glycoproteins of interest using both selective and/or combined elution.",
keywords = "Affinity chromatography, Electrophoresis, Lectin, Pseudo-lectin",
author = "Alex Monzo and Bonn, {Guenther K.} and A. Guttman",
year = "2007",
month = "12",
doi = "10.1007/s00216-007-1627-y",
language = "English",
volume = "389",
pages = "2097--2102",
journal = "Fresenius Zeitschrift fur Analytische Chemie",
issn = "1618-2642",
publisher = "Springer Verlag",
number = "7-8",

}

TY - JOUR

T1 - Boronic acid-lectin affinity chromatography. 1. Simultaneous glycoprotein binding with selective or combined elution

AU - Monzo, Alex

AU - Bonn, Guenther K.

AU - Guttman, A.

PY - 2007/12

Y1 - 2007/12

N2 - We introduce a novel combination of boronic acid affinity chromatography with lectin affinity chromatography, dubbed as boronic acid-lectin affinity chromatography (BLAC). Concanavalin A and wheat germ agglutinin lectins were mixed with the pesudo-lectin boronic acid to form the BLAC affinity column and their performance was evaluated with standard glycoproteins. Optimization of the binding and elution buffers for the BLAC system is described. The BLAC columns were employed to isolate glycoproteins of interest using both selective and/or combined elution.

AB - We introduce a novel combination of boronic acid affinity chromatography with lectin affinity chromatography, dubbed as boronic acid-lectin affinity chromatography (BLAC). Concanavalin A and wheat germ agglutinin lectins were mixed with the pesudo-lectin boronic acid to form the BLAC affinity column and their performance was evaluated with standard glycoproteins. Optimization of the binding and elution buffers for the BLAC system is described. The BLAC columns were employed to isolate glycoproteins of interest using both selective and/or combined elution.

KW - Affinity chromatography

KW - Electrophoresis

KW - Lectin

KW - Pseudo-lectin

UR - http://www.scopus.com/inward/record.url?scp=36649037780&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=36649037780&partnerID=8YFLogxK

U2 - 10.1007/s00216-007-1627-y

DO - 10.1007/s00216-007-1627-y

M3 - Article

C2 - 17909756

AN - SCOPUS:36649037780

VL - 389

SP - 2097

EP - 2102

JO - Fresenius Zeitschrift fur Analytische Chemie

JF - Fresenius Zeitschrift fur Analytische Chemie

SN - 1618-2642

IS - 7-8

ER -