Bone regeneration after topical BMP-2-gene delivery in circumferential peri-implant bone defects

Rainer Lutz, Jung Park, E. Felszeghy, Jörg Wiltfang, Emeke Nkenke, Karl Andreas Schlegel

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Objectives: The aims of this study were to evaluate the rate of bone formation and osseointegration after topical gene delivery with a liposomal vector system carrying bone morphogenetic protein (BMP)-2 cDNA in combination with a collagen carrier and autologous bone as a carrier in freshly created peri-implant bone defects. Materials and methods: Eight domestic pigs received nine calvariae defects each (10 × 7 mm). A dental implant was inserted into the centre of each defect. In the test groups, the remaining space was filled with the liposomal vector/BMP-2 complex combined with a collagen carrier (n=18) or an autologous bone graft (n=18). Control groups were collagen only (n=18) and autologous bone graft only (n=18). Results: There was a significant difference in mineralisation rate in the BMP-2/bone graft (29.9% ± 4.8 and 68.3% ± 7.2) and bone graft only (22.6% ± 2.6 and 49.4% ± 13.9) groups after 7 and 28 days. Mineralisation values were also significantly higher in the BMP-2/collagen group (21.2% ± 16.2 and 53.1% ± 12.5) compared with the collagen-only group (8.2% ± 7 and 41% ± 8.1) in two different regions after 28 days. Also the bone-to-implant contact was significantly increased in the BMP-2/bone graft group after 28 days and in the BMP-2/collagen group after 7 and 28 days compared with their control groups. Conclusions: The results of this study show a significantly positive effect of liposomal vector/BMP-2 on bone regeneration and osseointegration in bony circumferential peri-implant defects.

Original languageEnglish
Pages (from-to)590-599
Number of pages10
JournalClinical Oral Implants Research
Volume19
Issue number6
DOIs
Publication statusPublished - Jun 2008

Fingerprint

Bone Morphogenetic Protein 2
Bone Regeneration
Bone and Bones
Collagen
Genes
Transplants
Osseointegration
Sus scrofa
Control Groups
Dental Implants
Osteogenesis
Skull
Complementary DNA

Keywords

  • Animal experiments
  • Bone regeneration
  • Bone-implant interactions
  • Genetics
  • Growth factors
  • Guided tissue regeneration

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Bone regeneration after topical BMP-2-gene delivery in circumferential peri-implant bone defects. / Lutz, Rainer; Park, Jung; Felszeghy, E.; Wiltfang, Jörg; Nkenke, Emeke; Schlegel, Karl Andreas.

In: Clinical Oral Implants Research, Vol. 19, No. 6, 06.2008, p. 590-599.

Research output: Contribution to journalArticle

Lutz, Rainer ; Park, Jung ; Felszeghy, E. ; Wiltfang, Jörg ; Nkenke, Emeke ; Schlegel, Karl Andreas. / Bone regeneration after topical BMP-2-gene delivery in circumferential peri-implant bone defects. In: Clinical Oral Implants Research. 2008 ; Vol. 19, No. 6. pp. 590-599.
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abstract = "Objectives: The aims of this study were to evaluate the rate of bone formation and osseointegration after topical gene delivery with a liposomal vector system carrying bone morphogenetic protein (BMP)-2 cDNA in combination with a collagen carrier and autologous bone as a carrier in freshly created peri-implant bone defects. Materials and methods: Eight domestic pigs received nine calvariae defects each (10 × 7 mm). A dental implant was inserted into the centre of each defect. In the test groups, the remaining space was filled with the liposomal vector/BMP-2 complex combined with a collagen carrier (n=18) or an autologous bone graft (n=18). Control groups were collagen only (n=18) and autologous bone graft only (n=18). Results: There was a significant difference in mineralisation rate in the BMP-2/bone graft (29.9{\%} ± 4.8 and 68.3{\%} ± 7.2) and bone graft only (22.6{\%} ± 2.6 and 49.4{\%} ± 13.9) groups after 7 and 28 days. Mineralisation values were also significantly higher in the BMP-2/collagen group (21.2{\%} ± 16.2 and 53.1{\%} ± 12.5) compared with the collagen-only group (8.2{\%} ± 7 and 41{\%} ± 8.1) in two different regions after 28 days. Also the bone-to-implant contact was significantly increased in the BMP-2/bone graft group after 28 days and in the BMP-2/collagen group after 7 and 28 days compared with their control groups. Conclusions: The results of this study show a significantly positive effect of liposomal vector/BMP-2 on bone regeneration and osseointegration in bony circumferential peri-implant defects.",
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