Bone marrow-derived mast cell differentiation is strongly reduced in histidine decarboxylase knockout, histamine-free mice

Zoltán Wiener, Márton Andrásfalvy, E. Pállinger, Péter Kovács, C. Szalai, A. Erdei, S. Tóth, András Nagy, A. Falus

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27 Citations (Scopus)

Abstract

Mast cells are differentiated in vitro from bone marrow precursors. In this study the development of bone marrow-derived mast cells was examined from histidine decarboxylase deficient (HDC-/-) and wild-type mice in the presence of IL-3. The number of non-adherent, tryptase- and c-kit-positive mast cells in bone marrow-derived cultures of HDC-/- mice was decreased compared to that of wild-type (HDC+/+) animals, but within the tryptase- and c-kit-positive cells there was no difference in the expression intensity of both markers between the two groups. Furthermore, less serine proteases mMCP5, mMCP6 and FcεRIα mRNA were detected in bone marrow-derived cell cultures originating from HDC-/- mice. Antigen-provoked degranulation through high-affinity Fcεl receptor was also lower in HDC-/- mice. The colony assays in semisolid medium yielded a significantly lower ratio of mixed colonies and higher proportion of macrophage colonies from HDC-/- mice-derived bone marrow compared to the wild-type. In the course of the differentiation of HDC-/- -derived mast cells exogenously added histamine is unable to substitute the endogenously missing histamine. Concordantly, α-fluoromethyl-histamine, the specific inhibitor of HDC, revealed only a marginal inhibition on the differentiation of tryptase-positive mast cells from wild-type mice. These findings suggest that the effect of histamine on the IL-3-dependent development of bone marrow-derived mast cell differentiation during the early period is crucial and irreplaceable.

Original languageEnglish
Pages (from-to)381-387
Number of pages7
JournalInternational Immunology
Volume14
Issue number4
Publication statusPublished - 2002

Fingerprint

Histidine Decarboxylase
Mast Cells
Histamine
Cell Differentiation
Bone Marrow
Tryptases
Interleukin-3
Wild Animals
Serine Proteases
Bone Marrow Cells
Cell Culture Techniques
Macrophages
Antigens
Messenger RNA

Keywords

  • Bone marrow
  • C-kit
  • Differentiation
  • Gene targeting
  • Histamine
  • ILMast cell
  • Tryptase

ASJC Scopus subject areas

  • Immunology

Cite this

Bone marrow-derived mast cell differentiation is strongly reduced in histidine decarboxylase knockout, histamine-free mice. / Wiener, Zoltán; Andrásfalvy, Márton; Pállinger, E.; Kovács, Péter; Szalai, C.; Erdei, A.; Tóth, S.; Nagy, András; Falus, A.

In: International Immunology, Vol. 14, No. 4, 2002, p. 381-387.

Research output: Contribution to journalArticle

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