Blood cells as a source of transcriptional biomarkers of childhood obesity and its related metabolic alterations

Results of the IDEFICS study

Juana Sánchez, Teresa Priego, Catalina Picó, Wolfgang Ahrens, Stefaan De Henauw, Arno Fraterman, Staffan Mårild, D. Molnár, Luis A. Moreno, Jenny Peplies, Paola Russo, Alfonso Siani, Michael Tornaritis, Toomas Veidebaum, Andreu Palou

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Background: IDEFICS (Identification and Prevention of Dietary- and Lifestyle-Induced Health Effects in Children and Infants Project) is a European multicenter study on childhood obesity. One of its goals is to define early biomarkers of risk associated with obesity and its comorbid conditions. Objective: We considered blood cells as a new potential source of transcriptional biomarkers for these metabolic disorders and examined whether blood cell mRNA levels of some selected genes (LEPR, INSR, CPT1A, SLC27A2, UCP2, FASN, and PPARα) were altered in overweight children and whether their expression levels could be defined as markers of the insulin-resistant or dyslipidemic state associated with overweight. Design: Blood samples were obtained from 306 normal-weight and overweight children, aged 2-9 yr, from eight different European countries. Whole-blood mRNA levels were assessed by quantitative RT-PCR. Results: LEPR, INSR, and CPT1A mRNA levels were higher in overweight compared with normal-weight children (the two latter only in males), whereas SLC27A2 mRNA levels were lower in overweight children. Significant associations were also found between expression levels of LEPR, INSR, CPT1A, SLC27A2, FASN, PPARα, and different parameters, including body mass index, homeostasis model assessment index, and plasma triglycerides and cholesterol levels. These associations showed that high expression levels of CPT1A, SLC27A2, INSR, FASN, or PPARα may be indicative of a lower risk for the insulin-resistant or dyslipidemic state associated with obesity, whereas low LEPR mRNA levels appear as a marker of high low-density lipoprotein cholesterol, independently of body mass index. Conclusions: These findings point toward the possibility of using the expression levels of these genes in blood cells as markers of metabolic status and can potentially provide an early warning of a future disorder.

Original languageEnglish
JournalJournal of Clinical Endocrinology and Metabolism
Volume97
Issue number4
DOIs
Publication statusPublished - Apr 2012

Fingerprint

Pediatric Obesity
Biomarkers
Blood Cells
Blood
Cells
Peroxisome Proliferator-Activated Receptors
Messenger RNA
Body Mass Index
Obesity
Genes
Insulin
Weights and Measures
LDL Cholesterol
HDL Cholesterol
Multicenter Studies
Life Style
Triglycerides
Homeostasis
Cholesterol
Health

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Endocrinology
  • Biochemistry, medical
  • Endocrinology, Diabetes and Metabolism

Cite this

Blood cells as a source of transcriptional biomarkers of childhood obesity and its related metabolic alterations : Results of the IDEFICS study. / Sánchez, Juana; Priego, Teresa; Picó, Catalina; Ahrens, Wolfgang; De Henauw, Stefaan; Fraterman, Arno; Mårild, Staffan; Molnár, D.; Moreno, Luis A.; Peplies, Jenny; Russo, Paola; Siani, Alfonso; Tornaritis, Michael; Veidebaum, Toomas; Palou, Andreu.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 97, No. 4, 04.2012.

Research output: Contribution to journalArticle

Sánchez, J, Priego, T, Picó, C, Ahrens, W, De Henauw, S, Fraterman, A, Mårild, S, Molnár, D, Moreno, LA, Peplies, J, Russo, P, Siani, A, Tornaritis, M, Veidebaum, T & Palou, A 2012, 'Blood cells as a source of transcriptional biomarkers of childhood obesity and its related metabolic alterations: Results of the IDEFICS study', Journal of Clinical Endocrinology and Metabolism, vol. 97, no. 4. https://doi.org/10.1210/jc.2011-2209
Sánchez, Juana ; Priego, Teresa ; Picó, Catalina ; Ahrens, Wolfgang ; De Henauw, Stefaan ; Fraterman, Arno ; Mårild, Staffan ; Molnár, D. ; Moreno, Luis A. ; Peplies, Jenny ; Russo, Paola ; Siani, Alfonso ; Tornaritis, Michael ; Veidebaum, Toomas ; Palou, Andreu. / Blood cells as a source of transcriptional biomarkers of childhood obesity and its related metabolic alterations : Results of the IDEFICS study. In: Journal of Clinical Endocrinology and Metabolism. 2012 ; Vol. 97, No. 4.
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AU - Ahrens, Wolfgang

AU - De Henauw, Stefaan

AU - Fraterman, Arno

AU - Mårild, Staffan

AU - Molnár, D.

AU - Moreno, Luis A.

AU - Peplies, Jenny

AU - Russo, Paola

AU - Siani, Alfonso

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