Blood-brain barrier impairment by low pH buffer perfusion via the internal carotid artery in rat

Z. Nagy, M. Szabó, I. Hüttner

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Krebs-Ringer bicarbonate buffer (KRB) adjusted by lactic acid to low pH levels (6.6, 6.2, 6.0, 5.8, 5.5) was perfused via the internal carotid artery as a bolus into rat brain hemispheres. In one group of animals, the fluid phase tracer horseradish peroxidase (HRP) was perfused immediately following the low pH treatment. In the other group of animals, the brain hemispheres were fixed and the endothelial cells were stained with colloidal iron (CI) at pH 1.8. Widespread extravasation of HRP was detected indicating blood-brain barrier (BBB) opening to this tracer in hemispheres perfused with KRB at pH 6.2, 6.0, 5.8, or 5.5. HRP was seen in pools of endothelial tight junctions. Endothelial cell injury reflected by swelling and influx of HRP into the cytoplasm was occasionally encountered. CI evenly decorated the negatively charged surface of endothelial cells in the control brains, in contrast to markedly diminished iron binding capacity of endothelial cells in low pH-treated hemispheres. Our results suggest that the ionic milieu influences the negatively charged cell surface sialoglycoproteins and glycolipids, which are integral parts of the BBB system.

Original languageEnglish
Pages (from-to)160-163
Number of pages4
JournalActa Neuropathologica
Volume68
Issue number2
DOIs
Publication statusPublished - Jun 1985

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Internal Carotid Artery
Blood-Brain Barrier
Horseradish Peroxidase
Buffers
Perfusion
Endothelial Cells
Iron
Bicarbonates
Brain
Sialoglycoproteins
Tight Junctions
Glycolipids
Lactic Acid
Cytoplasm
Wounds and Injuries

Keywords

  • Cerebral endothelium
  • Colloidal iron staining at pH 1.8
  • Endothelial surface coat
  • Horseradish peroxidase
  • Low pH buffer perfusion

ASJC Scopus subject areas

  • Neuroscience(all)
  • Pathology and Forensic Medicine
  • Clinical Neurology

Cite this

Blood-brain barrier impairment by low pH buffer perfusion via the internal carotid artery in rat. / Nagy, Z.; Szabó, M.; Hüttner, I.

In: Acta Neuropathologica, Vol. 68, No. 2, 06.1985, p. 160-163.

Research output: Contribution to journalArticle

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