Blood-brain barrier changes during compensated and decompensated hemorrhagic shock

I. Krizbai, Gabor Lenzser, Erzsebet Szatmari, Attila E. Farkas, I. Wilhelm, Zsyzsanna Fekete, Benedek Erdos, Hannelore Bauer, Hans Christian Bauer, Peter Sandor, K. Komjáti

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Dysfunction of the blood-brain barrier (BBB) can be associated with a large number of central nervous system and systemic disorders. The aim of the present study was to determine BBB changes during different phases of hemorrhagic shock. The experiments were carried out on male Wistar rats anaesthetized with urethane. To produce compensated or decompensated hemorrhagic shock, mean arterial pressure was decreased from the normotensive control values to 40 mmHg by a standardized method of blood withdrawal from the femoral artery. Cerebral blood flow changes were followed by laser-Doppler flowmetry, and arterial blood gas values were monitored over the whole procedure. Cortical blood flow was significantly reduced in compensated and in decompensated hemorrhagic shock compared with the normotensive rats. As the shock shifted to the decompensated phase, the blood flow reduction was more pronounced. BBB permeability studies using sodium fluorescein (molecular weight of 376) and Evan's Blue albumin (molecular weight of 67,000) have revealed a significant increase of the BBB permeability for sodium fluorescein in the decompensated stage of hemorrhagic shock. Western blot analysis of brain capillaries showed that the expression of the transmembrane tight junction protein occludin was reduced in response to hemorrhagic shock, and the decrease of occludin was more pronounced in the decompensated phase. A similar expression pattern was shown by the transmembrane adherens junction protein cadherin as well. Our results suggest that the decompensated phase of hemorrhagic shock is associated with disturbances of the BBB, which may be explained by the dysfunction of interendothelial junctions caused by decreased occludin and cadherin levels.

Original languageEnglish
Pages (from-to)428-433
Number of pages6
JournalShock
Volume24
Issue number5
DOIs
Publication statusPublished - Nov 2005

Fingerprint

Hemorrhagic Shock
Blood-Brain Barrier
Occludin
Cadherins
Fluorescein
Permeability
Cerebrovascular Circulation
Molecular Weight
Tight Junction Proteins
Adherens Junctions
Evans Blue
Laser-Doppler Flowmetry
Central Nervous System Diseases
Urethane
Femoral Artery
Wistar Rats
Albumins
Shock
Arterial Pressure
Gases

Keywords

  • Blood-brain barrier
  • Cadherin
  • Hemorrhagic shock
  • Occludin
  • Permeability
  • Tight junction

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine
  • Physiology

Cite this

Blood-brain barrier changes during compensated and decompensated hemorrhagic shock. / Krizbai, I.; Lenzser, Gabor; Szatmari, Erzsebet; Farkas, Attila E.; Wilhelm, I.; Fekete, Zsyzsanna; Erdos, Benedek; Bauer, Hannelore; Bauer, Hans Christian; Sandor, Peter; Komjáti, K.

In: Shock, Vol. 24, No. 5, 11.2005, p. 428-433.

Research output: Contribution to journalArticle

Krizbai, I, Lenzser, G, Szatmari, E, Farkas, AE, Wilhelm, I, Fekete, Z, Erdos, B, Bauer, H, Bauer, HC, Sandor, P & Komjáti, K 2005, 'Blood-brain barrier changes during compensated and decompensated hemorrhagic shock', Shock, vol. 24, no. 5, pp. 428-433. https://doi.org/10.1097/01.shk.0000183389.98519.d3
Krizbai, I. ; Lenzser, Gabor ; Szatmari, Erzsebet ; Farkas, Attila E. ; Wilhelm, I. ; Fekete, Zsyzsanna ; Erdos, Benedek ; Bauer, Hannelore ; Bauer, Hans Christian ; Sandor, Peter ; Komjáti, K. / Blood-brain barrier changes during compensated and decompensated hemorrhagic shock. In: Shock. 2005 ; Vol. 24, No. 5. pp. 428-433.
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