Blockade of nitric oxide synthase inhibits nerve-mediated contraction in the rat small intestine

L. Barthó, G. Kóczán, G. Pethö, C. A. Maggi

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Abstract

Inhibitors of nitric oxide (NO) synthase inhibit nerve-mediated non-adrenergic, non-cholinergic (NANC) smooth muscle relaxation in the gastrointestinal tract. In this study, the effect of a NO synthase inhibitor, NG-nitro-l-arginine, was examined on the tetrodotoxin-sensitive NANC c response of the rat isolated ileal myenteric plexus-longitudinal muscle evoked by electrical field stimulation. This contraction was concentration-depe inhibited by NG-nitro-l-arginine (1-3×10-5 M). The inhibition was partly or entirely reversed by l-arginine. The did not exhibit any non-specific smooth-muscle depressant action or local anaesthetic effect. Sodium nitroprusside, a putative donor of NO also caused a transient contraction of the rat ileal strip. This response was resistant to tetrodotoxin. It is concluded that an NO synthase product is involved in the mechanism of the nerve-mediated NANC primary contraction due to field stimulation in the rat small intestine.

Original languageEnglish
Pages (from-to)43-46
Number of pages4
JournalNeuroscience Letters
Volume145
Issue number1
DOIs
Publication statusPublished - Sep 28 1992

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Keywords

  • Enteric neuron
  • Nitric oxide
  • Rat ileum
  • Smooth muscle contraction

ASJC Scopus subject areas

  • Neuroscience(all)

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