Birth and clinical pregnancy from fresh and frozen oocytes fertilized with cryopreserved testicular spermatozoa

József Zeke, János Konc, Katalin Kanyo, Rita Kriston, Sandor Cseh

Research output: Contribution to journalArticle

5 Citations (Scopus)


This is the first report showing a second clinical pregnancy of a couple who already have a baby from a previous frozen embryo transfer cycle when the embryos were generated from fresh oocytes that were fertilized by intracytoplasmic sperm injection (ICSI) using frozen testicular spermatozoa (the couple have unsuccessful fresh and frozen embryo transfer cycles). Fifty-two months after the first IVFICSI cycle the couple had their second IVFICSI cycle, but the collected oocytes (n8) were frozen because no spermatozoa was obtained from the frozen testicular tissue samples which were cryopreserved prior to the first IVFICSI cycle. New testicular tissue samples were obtained and frozen. Finally, 58 months after the first IVFICSI cycle all of the 8 frozen oocytes of the couple were thawed and fertilized by ICSI using frozen testicular spermatozoa obtained from the newly cryopreserved testicular tissue. Three embryos were transferred and the couple has an ongoing pregnancy, which is in the 20th week of pregnancy. Our case report shows that: 1) developmentally competent embryos can be generated by ICSI of frozen-thawed testicular spermatozoa into both fresh and frozen human oocytes, and 2) clinical pregnancy and a healthy baby can be conceived from both frozen and fresh oocytes fertilized with cryopreserved testicular spermatozoa. ©

Original languageEnglish
Pages (from-to)165-167
Number of pages3
JournalSystems Biology in Reproductive Medicine
Issue number3
Publication statusPublished - Jun 1 2012



  • Embryo freezing
  • Frozen testicular spermatozoa
  • ICSI
  • Intracytoplasmic sperm injection
  • Oocyte cryopreservation
  • Testicular sperm extraction

ASJC Scopus subject areas

  • Reproductive Medicine
  • Urology

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