Bipolar filaments of human nonmuscle myosin 2-A and 2-B have distinct motile and mechanical properties

Luca Melli, Neil Billington, Sara A. Sun, Jonathan E. Bird, A. Nagy, Thomas B. Friedman, Yasuharu Takagi, James R. Sellers

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Nonmusclemyosin 2 (NM-2) powers cell motility and tissue morphogenesis by assembling into bipolar filaments that interact with actin. Although the enzymatic properties of purified NM-2 motor fragments have been determined, the emergent properties of filament ensembles are unknown. Using single myosin filament in vitro motility assays, we report fundamental differences in filaments formed of different NM-2 motors. Filaments consisting of NM2-B moved processively along actin, while under identical conditions, NM2-A filaments did not. By more closely mimicking the physiological milieu, either by increasing solution viscosity or by copolymerization with NM2-B, NM2-A containing filaments moved processively. Our data demonstrate that both the kinetic and mechanical properties of these two myosins, in addition to the stochiometry of NM-2 subunits, can tune filament mechanical output. We propose altering NM- 2 filament composition is a general cellular strategy for tailoring force production of filaments to specific functions, such as maintaining tension or remodeling actin.

Original languageEnglish
Article numbere32871
JournaleLife
Volume7
DOIs
Publication statusPublished - Feb 8 2018

Fingerprint

Myosins
Actins
Mechanical properties
Morphogenesis
Viscosity
Copolymerization
Cell Movement
Assays
Tissue
Kinetics
Chemical analysis

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Melli, L., Billington, N., Sun, S. A., Bird, J. E., Nagy, A., Friedman, T. B., ... Sellers, J. R. (2018). Bipolar filaments of human nonmuscle myosin 2-A and 2-B have distinct motile and mechanical properties. eLife, 7, [e32871]. https://doi.org/10.7554/eLife.32871

Bipolar filaments of human nonmuscle myosin 2-A and 2-B have distinct motile and mechanical properties. / Melli, Luca; Billington, Neil; Sun, Sara A.; Bird, Jonathan E.; Nagy, A.; Friedman, Thomas B.; Takagi, Yasuharu; Sellers, James R.

In: eLife, Vol. 7, e32871, 08.02.2018.

Research output: Contribution to journalArticle

Melli, L, Billington, N, Sun, SA, Bird, JE, Nagy, A, Friedman, TB, Takagi, Y & Sellers, JR 2018, 'Bipolar filaments of human nonmuscle myosin 2-A and 2-B have distinct motile and mechanical properties', eLife, vol. 7, e32871. https://doi.org/10.7554/eLife.32871
Melli, Luca ; Billington, Neil ; Sun, Sara A. ; Bird, Jonathan E. ; Nagy, A. ; Friedman, Thomas B. ; Takagi, Yasuharu ; Sellers, James R. / Bipolar filaments of human nonmuscle myosin 2-A and 2-B have distinct motile and mechanical properties. In: eLife. 2018 ; Vol. 7.
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