Biphasic effect of prostaglandin E1 on the severity of acute pancreatitis induced by a closed duodenal loop in rats

J. Pozsár, Z. Berger, K. Simon, A. Kovácsai, E. Marosi, Á Pap

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The effect of prostaglandin E1 (PGE1) on the severity of acute pancreatitis induced by a closed duodenal loop in the rat was tested. PGE1 was administered subcutaneously at various doses (3, 6, 12, and 24 μg/kg) at hourly intervals, from the induction of acute pancreatitis up to the 24th hour. A saline-treated group served as the control. The mortality rate was recorded, and pancreatic histology was evaluated by a scoring system. Serum amylase activity and pancreatic amylase, trypsin, protein, and desoxyribonucleic acid (DNA) contents were determined at 24 h. Administration of PGE1 influenced the severity of acute pancreatitis biphasically. Serum amylase was reduced significantly (p < 0.01) at a dose of 12 μg/kg/h, but less at 24 μg/kg/h. Pancreatic weights did not differ in the groups. Pancreatic amylase, trypsin, and protein contents showed significant elevations (p < 0.01), yet the mortality rate was reduced using 6 and 12 μg/kg/h doses of PGE1 compared to controls, but not at higher and lower doses. The DNA content was significantly higher at the 6 μg/kg/h dose, compared to the control. The extent of necrosis and the severity of hemorrhage were reduced significantly (p < 0.05) at doses of 6 and 12 μg/kg/h of PGE1, but less at 24 μg/kg/h. Leukocyte infiltration was not affected. In conclusion, optimal doses of PGE1 can ameliorate, but higher doses increase, the severity of acute pancreatitis in this experimental model.

Original languageEnglish
Pages (from-to)159-164
Number of pages6
JournalPancreas
Volume12
Issue number2
DOIs
Publication statusPublished - Jan 1 1996

Keywords

  • Acute pancreatitis
  • Closed duodenal loop
  • Prostaglandin E
  • Rat

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism
  • Hepatology
  • Endocrinology

Fingerprint Dive into the research topics of 'Biphasic effect of prostaglandin E<sub>1</sub> on the severity of acute pancreatitis induced by a closed duodenal loop in rats'. Together they form a unique fingerprint.

  • Cite this