Biphasic calcium response of platelet-derived growth factor stimulated glioblastoma cells is a function of cell confluence

G. Vereb, Burt G. Feuerstein, William C. Hyun, Mack J. Fulwyler, M. Balázs, J. Szöllősi

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Background: Previous reports have linked the spiking or two-phased character of calcium transients evoked by platelet-derived growth factor (PDGF) to the position of cells in the cell cycle without regard to cell-cell contact and communication. Because cell confluence can regulate growth factor receptor expression and dephosphorylation, we investigated the effect of cell culture confluence and cell cycle on calcium responses of PDGF-BB-stimulated A172 glioblastoma cells. Methods: Digital imaging cytometry was used to correlate the peak and duration of calcium response with bromodeoxyuridine positivity and DNA content and with culture confluence on a cell-by-cell basis. Results: In serum-starved cultures, complete two-phase calcium signals and shorter, lower spikes occurred independent of cell cycle phase. However, the confluence of cell culture seemed essential for inducing a complete response because cells in sparse cultures exhibited mostly short spikes with lower peaks or no transients at all. Conclusion: Because cell confluence, by virtue of cell-cell contacts, is assumed to be an important regulator of proliferation, one is tempted to speculate that in transformed cells the ability to produce stronger growth signals upon reaching confluence and facing contact inhibition could provide a proliferative advantage.

Original languageEnglish
Pages (from-to)172-179
Number of pages8
JournalCytometry Part A
Volume67
Issue number2
DOIs
Publication statusPublished - Oct 2005

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Platelet-Derived Growth Factor
Glioblastoma
Calcium
Cell Cycle
Cell Culture Techniques
Contact Inhibition
Growth Factor Receptors
Bromodeoxyuridine
Cell Communication

Keywords

  • Bromodeoxyuridine incorporation
  • Calcium signaling
  • Cell confluence
  • Cell cycle
  • Cell-cell contact
  • Digital imaging cytometry
  • Glioblastoma
  • I
  • Intracellular release
  • Platelet-derived growth factor receptor
  • Store-operated influx

ASJC Scopus subject areas

  • Hematology
  • Cell Biology
  • Pathology and Forensic Medicine
  • Biophysics
  • Endocrinology

Cite this

Biphasic calcium response of platelet-derived growth factor stimulated glioblastoma cells is a function of cell confluence. / Vereb, G.; Feuerstein, Burt G.; Hyun, William C.; Fulwyler, Mack J.; Balázs, M.; Szöllősi, J.

In: Cytometry Part A, Vol. 67, No. 2, 10.2005, p. 172-179.

Research output: Contribution to journalArticle

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