Biotechnological intensification of biogas production

Zoltán Bagi, Norbert Ács, Balázs Bálint, Lenke Horváth, Krisztina Dobó, Katalin R. Perei, G. Rákhely, K. Kovács

Research output: Contribution to journalArticle

94 Citations (Scopus)

Abstract

The importance of syntrophic relationships among microorganisms participating in biogas formation has been emphasized, and the regulatory role of in situ hydrogen production has been recognized. It was assumed that the availability of hydrogen may be a limiting factor for hydrogenotrophic methanogens. This hypothesis was tested under laboratory and field conditions by adding a mesophilic (Enterobacter cloacae) or thermophilic hydrogen-producing (Caldicellulosyruptor saccharolyticus) strain to natural biogas-producing consortia. The substrates were waste water sludge, dried plant biomass from Jerusalem artichoke, and pig manure. In all cases, a significant intensification of biogas production was observed. The composition of the generated biogas did not noticeably change. In addition to being a good hydrogen producer, C. saccharolyticus has cellulolytic activity; hence, it is particularly suitable when cellulose-containing biomass is fermented. The process was tested in a 5-m3 thermophilic biogas digester using pig manure slurry as a substrate. Biogas formation increased at least 160-170% upon addition of the hydrogen-producing bacteria as compared to the biogas production of the spontaneously formed microbial consortium. Using the hydrogenase-minus control strain provided evidence that the observed enhancement was due to interspecies hydrogen transfer. The on-going presence of C. saccharolyticus was demonstrated after several months of semicontinuous operation.

Original languageEnglish
Pages (from-to)473-482
Number of pages10
JournalApplied Microbiology and Biotechnology
Volume76
Issue number2
DOIs
Publication statusPublished - Aug 2007

Fingerprint

Biofuels
Biogas
Hydrogen
Manure
Manures
Biomass
Swine
Microbial Consortia
Strain control
Methanogens
Hydrogenase
Enterobacter cloacae
Helianthus
Substrates
Hydrogen production
Waste Water
Sewage
Cellulose
Microorganisms
Bacteria

Keywords

  • Biogas
  • Hydrogen
  • Intensification
  • Interspecies electron transfer
  • Syntrophy
  • Thermophilic

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology
  • Bioengineering
  • Microbiology (medical)

Cite this

Biotechnological intensification of biogas production. / Bagi, Zoltán; Ács, Norbert; Bálint, Balázs; Horváth, Lenke; Dobó, Krisztina; Perei, Katalin R.; Rákhely, G.; Kovács, K.

In: Applied Microbiology and Biotechnology, Vol. 76, No. 2, 08.2007, p. 473-482.

Research output: Contribution to journalArticle

Bagi Z, Ács N, Bálint B, Horváth L, Dobó K, Perei KR et al. Biotechnological intensification of biogas production. Applied Microbiology and Biotechnology. 2007 Aug;76(2):473-482. https://doi.org/10.1007/s00253-007-1009-6
Bagi, Zoltán ; Ács, Norbert ; Bálint, Balázs ; Horváth, Lenke ; Dobó, Krisztina ; Perei, Katalin R. ; Rákhely, G. ; Kovács, K. / Biotechnological intensification of biogas production. In: Applied Microbiology and Biotechnology. 2007 ; Vol. 76, No. 2. pp. 473-482.
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