Biosynthesized silver and gold nanoparticles are potent antimycotics against opportunistic pathogenic yeasts and dermatophytes

Andrea Rónavári, Nóra Igaz, Mohana Krishna Gopisetty, Bettina Szerencsés, Dávid Kovács, Csaba Papp, C. Vágvölgyi, I. Boros, Z. Kónya, Mónika Kiricsi, I. Pfeiffer

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Background: Epidemiologic observations indicate that the number of systemic fungal infections has increased significantly during the past decades, however in human mycosis, mainly cutaneous infections predominate, generating major public health concerns and providing much of the impetus for current attempts to develop novel and efficient agents against cutaneous mycosis causing species. Innovative, environmentally benign and economic nanotechnology-based approaches have recently emerged utilizing principally biological sources to produce nano-sized structures with unique antimicrobial properties. In line with this, our aim was to generate silver nanoparticles (AgNPs) and gold nanoparticles (AuNPs) by biological synthesis and to study the effect of the obtained nanoparticles on cutaneous mycosis causing fungi and on human keratinocytes. Methods: Cell-free extract of the red yeast Phaffia rhodozyma proved to be suitable for nanoparticle preparation and the generated AgNPs and AuNPs were characterized by transmission electron microscopy, dynamic light scattering and X-ray powder diffraction. Results: Antifungal studies demonstrated that the biosynthesized silver particles were able to inhibit the growth of several opportunistic Candida or Cryptococcus species and were highly potent against filamentous Microsporum and Trichophyton dermatophytes. Among the tested species only Cryptococcus neoformans was susceptible to both AgNPs and AuNPs. Neither AgNPs nor AuNPs exerted toxicity on human keratinocytes. Conclusion: Our results emphasize the therapeutic potential of such biosynthesized nanoparticles, since their biocompatibility to skin cells and their outstanding antifungal performance can be exploited for topical treatment and prophylaxis of superficial cutaneous mycosis.

Original languageEnglish
Pages (from-to)695-703
Number of pages9
JournalInternational Journal of Nanomedicine
Volume13
DOIs
Publication statusPublished - Feb 1 2018

Fingerprint

Arthrodermataceae
Silver
Gold
Yeast
Nanoparticles
Yeasts
Mycoses
Skin
Keratinocytes
Microsporum
Powder Diffraction
Cryptococcus
Trichophyton
Cryptococcus neoformans
Nanotechnology
Candida
Public health
Dynamic light scattering
Cell Extracts
Fungi

Keywords

  • Antifungal activity
  • Biological synthesis
  • Dermatophytes
  • Opportunistic pathogenic yeasts
  • Silver nanoparticles
  • Toxicity

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Drug Discovery
  • Organic Chemistry

Cite this

Biosynthesized silver and gold nanoparticles are potent antimycotics against opportunistic pathogenic yeasts and dermatophytes. / Rónavári, Andrea; Igaz, Nóra; Gopisetty, Mohana Krishna; Szerencsés, Bettina; Kovács, Dávid; Papp, Csaba; Vágvölgyi, C.; Boros, I.; Kónya, Z.; Kiricsi, Mónika; Pfeiffer, I.

In: International Journal of Nanomedicine, Vol. 13, 01.02.2018, p. 695-703.

Research output: Contribution to journalArticle

Rónavári, Andrea ; Igaz, Nóra ; Gopisetty, Mohana Krishna ; Szerencsés, Bettina ; Kovács, Dávid ; Papp, Csaba ; Vágvölgyi, C. ; Boros, I. ; Kónya, Z. ; Kiricsi, Mónika ; Pfeiffer, I. / Biosynthesized silver and gold nanoparticles are potent antimycotics against opportunistic pathogenic yeasts and dermatophytes. In: International Journal of Nanomedicine. 2018 ; Vol. 13. pp. 695-703.
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AU - Kovács, Dávid

AU - Papp, Csaba

AU - Vágvölgyi, C.

AU - Boros, I.

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AU - Kiricsi, Mónika

AU - Pfeiffer, I.

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AB - Background: Epidemiologic observations indicate that the number of systemic fungal infections has increased significantly during the past decades, however in human mycosis, mainly cutaneous infections predominate, generating major public health concerns and providing much of the impetus for current attempts to develop novel and efficient agents against cutaneous mycosis causing species. Innovative, environmentally benign and economic nanotechnology-based approaches have recently emerged utilizing principally biological sources to produce nano-sized structures with unique antimicrobial properties. In line with this, our aim was to generate silver nanoparticles (AgNPs) and gold nanoparticles (AuNPs) by biological synthesis and to study the effect of the obtained nanoparticles on cutaneous mycosis causing fungi and on human keratinocytes. Methods: Cell-free extract of the red yeast Phaffia rhodozyma proved to be suitable for nanoparticle preparation and the generated AgNPs and AuNPs were characterized by transmission electron microscopy, dynamic light scattering and X-ray powder diffraction. Results: Antifungal studies demonstrated that the biosynthesized silver particles were able to inhibit the growth of several opportunistic Candida or Cryptococcus species and were highly potent against filamentous Microsporum and Trichophyton dermatophytes. Among the tested species only Cryptococcus neoformans was susceptible to both AgNPs and AuNPs. Neither AgNPs nor AuNPs exerted toxicity on human keratinocytes. Conclusion: Our results emphasize the therapeutic potential of such biosynthesized nanoparticles, since their biocompatibility to skin cells and their outstanding antifungal performance can be exploited for topical treatment and prophylaxis of superficial cutaneous mycosis.

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