Biosynthesis of lipooligosaccharide nodulation factors: Rhizobium NodA protein is involved in N-acylation of the chitooligosaccharide backbone

Horst Röhrig, Jürgen Schmidt, Ursula Wieneke, Eva Kondorosi, Isabelle Barlier, Jeff Schell, Michael John

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Rhizobium meliloti interacts symbiotically with alfalfa by forming root nodules in which the bacteria fix nitrogen. The Rhizobium nodulation genes nodABC are involved in the synthesis of lipooligosaccharide symbiotic signal molecules, which are mono-N-acylated chitooligosaccharides. These bacterial signals elicit nodule organogenesis in roots of legumes. To elucidate the role of the NodA protein in lipooligosaccharide biosynthesis, we prepared a radiolabeled tetrasaccharide precursor carrying an amino group as a potential attachment site for N-acylation at the nonreducing glucosamine residue. Various criteria demonstrate that NodA is involved in the attachment of a fatty acyl chain to this tetrasaccharide precursor, yielding a biologically active nodulation factor.

Original languageEnglish
Pages (from-to)3122-3126
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume91
Issue number8
DOIs
Publication statusPublished - Apr 12 1994

Keywords

  • Rhizobium meliloti
  • common nod genes
  • nitrogen fixation

ASJC Scopus subject areas

  • General

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