Biosensors for the rapid detection of dopamine using bilayer lipid membranes (BLMs) with incorporated calix[4]resorcinarene receptor

Dimitrios P. Nikolelis, Syrago Styliani E. Petropoulou, Eva Pergel, Klara Toth

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10 Citations (Scopus)


This work explores the use of a calix[4]resorcinarene receptor (lipophilic macrocyclic host molecule) for the rapid electrochemical detection of dopamine using planar bilayer lipid membranes (BLMs). BLMs were composed of egg phosphatidylcholine (PC) and 35% (w/w) dipalmitoyl phosphatidic acid in which the receptor was incorporated. Freely-suspended and metal supported BLMs modified with the resorcin[4]arene receptor were used as one shot sensors to rapidly detect this catecholamine. The interactions of this compound with freely-suspended BLMs were found to be electrochemically transduced in the form of a transient current signal with duration of seconds, which reproducibly appeared within 8 s after exposure of the membranes to dopamine. The response time for these BLMs without incorporated receptor for dopamine was about 3 min. The magnitude of the transient current signal was related to the concentration of the stimulating agent in bulk solution in the micromolar range. The present electrochemical technique was also applied for the rapid and sensitive screening of dopamine using surface-stabilized bilayer lipid membranes (sBLMs). The interactions of dopamine with sBLMs with incorporated receptor produced electrochemical ion current increases, which reproducibly appeared within a few seconds after exposure of the membranes to the stimulant. The use of the receptor in sBLMs increased the sensitivity of the method by 10-fold. The current signal increases were related to the concentration of dopamine in bulk solution in the micromolar range. No interferences from ascorbic acid were noticed because of the use of the negatively charged lipids in membranes. The present technique can be used as one-shot sensor for the rapid detection of these pharmaceutical substances and keep prospects for the selective determination of catecholamines in biofluids.

Original languageEnglish
Pages (from-to)783-789
Number of pages7
Issue number11
Publication statusPublished - Jul 16 2002



  • Bilayer lipid membranes
  • Biosensors
  • Dopamine
  • Electrochemistry
  • Resorcin[4]arene receptor

ASJC Scopus subject areas

  • Analytical Chemistry
  • Electrochemistry

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