Biorelevant reactions of the potential anti-tumor agent vanadocene dichloride

Daniele Sanna, Maria Serra, Valeria Ugone, Laura Manca, Monica Pirastru, P. Buglyó, Linda Bíró, Giovanni Micera, Eugenio Garribba

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The interaction of the potential anti-tumor agent vanadocene dichloride ([Cp2VCl2] or VDC) with some relevant bioligands of the cytosol such as proteins (Hb), amino acids (glycine and histidine), NADH derivatives (NADH, NADPH, NAD+ and NADP+), reductants (GSH and ascorbic acid), phosphates (HPO4 2-, P2O7 4-, cAMP, AMP, ADP and ATP) and carboxylate derivatives (lactate) and its uptake by red blood cells were studied. The results indicated that [Cp2VCl2] transforms at physiological pH into [Cp2V(OH)2] and that only HPO4 2-, P2O7 4-, lactate, ATP and ADP form mixed species with the [Cp2V]2+ moiety replacing the two hydroxide ions. EPR and electronic absorption spectroscopy, agarose gel electrophoresis and spin trapping measurements allow excluding any direct interaction and/or intercalation with DNA and the formation of reactive oxygen species (ROS) in Fenton-like reactions. Uptake experiments by erythrocytes suggested that VDC crosses the membrane and enters inside the cells, whereas 'bare' VIV transforms into VIVO species with loss of the two cyclopentadienyl rings. This transformation in the cellular environment could be related to the mechanism of action of VDC.

Original languageEnglish
Pages (from-to)532-541
Number of pages10
JournalMetallomics
Volume8
Issue number5
DOIs
Publication statusPublished - May 1 2016

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Administrative data processing
Adenosinetriphosphate
NAD
Amino acids
Tumors
Derivatives
NADP
Adenosine Diphosphate
Ascorbic acid
Lactic Acid
Intercalation
Adenosine Triphosphate
Erythrocytes
Electrophoresis
Absorption spectroscopy
Spin Trapping
Paramagnetic resonance
Neoplasms
Phosphates
DNA

ASJC Scopus subject areas

  • Biomaterials
  • Metals and Alloys
  • Chemistry (miscellaneous)
  • Biochemistry
  • Biophysics

Cite this

Sanna, D., Serra, M., Ugone, V., Manca, L., Pirastru, M., Buglyó, P., ... Garribba, E. (2016). Biorelevant reactions of the potential anti-tumor agent vanadocene dichloride. Metallomics, 8(5), 532-541. https://doi.org/10.1039/c6mt00002a

Biorelevant reactions of the potential anti-tumor agent vanadocene dichloride. / Sanna, Daniele; Serra, Maria; Ugone, Valeria; Manca, Laura; Pirastru, Monica; Buglyó, P.; Bíró, Linda; Micera, Giovanni; Garribba, Eugenio.

In: Metallomics, Vol. 8, No. 5, 01.05.2016, p. 532-541.

Research output: Contribution to journalArticle

Sanna, D, Serra, M, Ugone, V, Manca, L, Pirastru, M, Buglyó, P, Bíró, L, Micera, G & Garribba, E 2016, 'Biorelevant reactions of the potential anti-tumor agent vanadocene dichloride', Metallomics, vol. 8, no. 5, pp. 532-541. https://doi.org/10.1039/c6mt00002a
Sanna, Daniele ; Serra, Maria ; Ugone, Valeria ; Manca, Laura ; Pirastru, Monica ; Buglyó, P. ; Bíró, Linda ; Micera, Giovanni ; Garribba, Eugenio. / Biorelevant reactions of the potential anti-tumor agent vanadocene dichloride. In: Metallomics. 2016 ; Vol. 8, No. 5. pp. 532-541.
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