Bioprocessing of sweet sorghum with in situ-produced enzymes

Robert P. Tengerdy, G. Szakács, Joźsef Sipocz

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Enzyme-assisted ensiling (ENLAC), using in situ-produced enzymes from Gliocladium sp. TUB-F-498, preserved 80% of the sugar content of sweet sorghum, and facilitated its extraction by countercurrent diffusion. The in situ enzyme was produced on the extracted sweet sorghum pulp by an 8-d solid substrate fermentation (SSF) with a yield of 4.6 cellulase and 400 IU/g dry wt xylanase. Two percent of the fermented substrate had cellulase and xylanase levels equivalent or superior to levels found in the commercial enzymes Celluclast and Viscozyme Novo at the 0.025% application level in ENLAC. The in situ-production of enzymes on recyclable substrates may reduce bioprocessing costs significantly. In this ENLAC process, the cost of the in situ enzymes is estimated to be about $0.12/MT substrate, compared to $9.5/metric ton (MT) for the commercial enzymes, a cost reduction of nearly 80-fold.

Original languageEnglish
Pages (from-to)563-569
Number of pages7
JournalApplied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
Volume57-58
Publication statusPublished - 1996

Fingerprint

Sorghum
Enzymes
Cellulase
Substrates
Costs and Cost Analysis
Gliocladium
Cost reduction
Sugars
Fermentation
Pulp
Costs

Keywords

  • Bioprocessing of sweet sorghum
  • Fungal cellulases and hemicellulases
  • In situ enzyme production

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Bioengineering

Cite this

Bioprocessing of sweet sorghum with in situ-produced enzymes. / Tengerdy, Robert P.; Szakács, G.; Sipocz, Joźsef.

In: Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology, Vol. 57-58, 1996, p. 563-569.

Research output: Contribution to journalArticle

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AB - Enzyme-assisted ensiling (ENLAC), using in situ-produced enzymes from Gliocladium sp. TUB-F-498, preserved 80% of the sugar content of sweet sorghum, and facilitated its extraction by countercurrent diffusion. The in situ enzyme was produced on the extracted sweet sorghum pulp by an 8-d solid substrate fermentation (SSF) with a yield of 4.6 cellulase and 400 IU/g dry wt xylanase. Two percent of the fermented substrate had cellulase and xylanase levels equivalent or superior to levels found in the commercial enzymes Celluclast and Viscozyme Novo at the 0.025% application level in ENLAC. The in situ-production of enzymes on recyclable substrates may reduce bioprocessing costs significantly. In this ENLAC process, the cost of the in situ enzymes is estimated to be about $0.12/MT substrate, compared to $9.5/metric ton (MT) for the commercial enzymes, a cost reduction of nearly 80-fold.

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