Biophysical studies of photosystem II-related recovery processes after a heat pulse in barley seedlings (Hordeum vulgare L.)

Szilvia Z. Tóth, G. Schansker, Judit Kissimon, László Kovács, G. Garab, Reto J. Strasser

Research output: Contribution to journalArticle

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Abstract

Leaves of 7-day-old barley seedlings were subjected to heat pulses at 50°C for 20 or 40 s to inhibit partially or fully the oxygen evolution without inducing visible symptoms. By means of biophysical techniques, we investigated the time course and mechanism of photosystem II (PSII) recovery. After the heat treatment, the samples were characterized by typical heat stress symptoms: loss of oxygen evolution activity, strong decrease of F v/Fm, induction of the K-step in the fluorescence induction transient, emergence of the AT-thermoluminescence-band and a dramatic increase in membrane permeability. In the first 4 h in the light following the heat pulse, the AT-band and the K-step disappeared in parallel, indicating the loss of this restricted activity of PSII. This phase was followed by a recovery period, during which PSII-activity was gradually restored in the light. In darkness, no recovery, except for the membrane permeability, was observed. A model is presented that accounts for (i) the damage induced by the heat pulse on the membrane architecture and on the PSII donor side, (ii) the light-dependent removal of the impaired reaction centers from the disorganized membrane, and (iii) the subsequent light-independent restoration of the membrane permeability and the de novo synthesis of the PSII reaction centers in the light.

Original languageEnglish
Pages (from-to)181-194
Number of pages14
JournalJournal of Plant Physiology
Volume162
Issue number2
DOIs
Publication statusPublished - Feb 21 2005

Fingerprint

Photosystem II Protein Complex
Hordeum
Seedlings
photosystem II
Hordeum vulgare
Hot Temperature
barley
heat
membrane permeability
Light
Membranes
seedlings
Permeability
signs and symptoms (plants)
Oxygen
Darkness
heat stress
Fluorescence
heat treatment
fluorescence

Keywords

  • Chlorophyll-a fluorescence induction
  • Heat stress
  • Photosystem II
  • Recovery

ASJC Scopus subject areas

  • Plant Science

Cite this

Biophysical studies of photosystem II-related recovery processes after a heat pulse in barley seedlings (Hordeum vulgare L.). / Tóth, Szilvia Z.; Schansker, G.; Kissimon, Judit; Kovács, László; Garab, G.; Strasser, Reto J.

In: Journal of Plant Physiology, Vol. 162, No. 2, 21.02.2005, p. 181-194.

Research output: Contribution to journalArticle

Tóth, Szilvia Z. ; Schansker, G. ; Kissimon, Judit ; Kovács, László ; Garab, G. ; Strasser, Reto J. / Biophysical studies of photosystem II-related recovery processes after a heat pulse in barley seedlings (Hordeum vulgare L.). In: Journal of Plant Physiology. 2005 ; Vol. 162, No. 2. pp. 181-194.
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