Bioorthogonal fluorescent labels: A review on combined forces

Gergely B. Cserép, András Herner, P. Kele

Research output: Contribution to journalReview article

25 Citations (Scopus)

Abstract

This review ventures to summarize the latest developments in bioorthogonal fluorescent imaging labels with a special focus on bioimaging applications. We briefly summarize the most preferred means of bioorthogonal tagging schemes for the labeling of specific biomolecular structures. The review is structured by the type of the fluorescent labels that can address the problems that most commonly compromise fluorescent imaging techniques, i.e. the autofluorescence of biomolecules, the background fluorescence of unreacted reagents, and photobleaching. Thus, we present (i) farred/ near-infra-red emitting dyes, (ii) fluorogenic scaffolds, and (iii) nanoparticle-based signaling platforms.

Original languageEnglish
Article number042001
JournalMethods and Applications in Fluorescence
Volume3
Issue number4
DOIs
Publication statusPublished - Dec 1 2015

Fingerprint

marking
Labels
Imaging techniques
Photobleaching
Biomolecules
Scaffolds
imaging techniques
Labeling
reagents
Coloring Agents
Dyes
platforms
dyes
Fluorescence
Nanoparticles
Infrared radiation
fluorescence
nanoparticles

Keywords

  • Bioimaging
  • Bioorthogonal
  • Fluorescent

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Instrumentation
  • Spectroscopy
  • Materials Science(all)

Cite this

Bioorthogonal fluorescent labels : A review on combined forces. / Cserép, Gergely B.; Herner, András; Kele, P.

In: Methods and Applications in Fluorescence, Vol. 3, No. 4, 042001, 01.12.2015.

Research output: Contribution to journalReview article

@article{bb89880679e146588f40cb9049a1cca2,
title = "Bioorthogonal fluorescent labels: A review on combined forces",
abstract = "This review ventures to summarize the latest developments in bioorthogonal fluorescent imaging labels with a special focus on bioimaging applications. We briefly summarize the most preferred means of bioorthogonal tagging schemes for the labeling of specific biomolecular structures. The review is structured by the type of the fluorescent labels that can address the problems that most commonly compromise fluorescent imaging techniques, i.e. the autofluorescence of biomolecules, the background fluorescence of unreacted reagents, and photobleaching. Thus, we present (i) farred/ near-infra-red emitting dyes, (ii) fluorogenic scaffolds, and (iii) nanoparticle-based signaling platforms.",
keywords = "Bioimaging, Bioorthogonal, Fluorescent",
author = "Cser{\'e}p, {Gergely B.} and Andr{\'a}s Herner and P. Kele",
year = "2015",
month = "12",
day = "1",
doi = "10.1088/2050-6120/3/4/042001",
language = "English",
volume = "3",
journal = "Methods and Applications in Fluorescence",
issn = "2050-6120",
publisher = "IOP Publishing Ltd.",
number = "4",

}

TY - JOUR

T1 - Bioorthogonal fluorescent labels

T2 - A review on combined forces

AU - Cserép, Gergely B.

AU - Herner, András

AU - Kele, P.

PY - 2015/12/1

Y1 - 2015/12/1

N2 - This review ventures to summarize the latest developments in bioorthogonal fluorescent imaging labels with a special focus on bioimaging applications. We briefly summarize the most preferred means of bioorthogonal tagging schemes for the labeling of specific biomolecular structures. The review is structured by the type of the fluorescent labels that can address the problems that most commonly compromise fluorescent imaging techniques, i.e. the autofluorescence of biomolecules, the background fluorescence of unreacted reagents, and photobleaching. Thus, we present (i) farred/ near-infra-red emitting dyes, (ii) fluorogenic scaffolds, and (iii) nanoparticle-based signaling platforms.

AB - This review ventures to summarize the latest developments in bioorthogonal fluorescent imaging labels with a special focus on bioimaging applications. We briefly summarize the most preferred means of bioorthogonal tagging schemes for the labeling of specific biomolecular structures. The review is structured by the type of the fluorescent labels that can address the problems that most commonly compromise fluorescent imaging techniques, i.e. the autofluorescence of biomolecules, the background fluorescence of unreacted reagents, and photobleaching. Thus, we present (i) farred/ near-infra-red emitting dyes, (ii) fluorogenic scaffolds, and (iii) nanoparticle-based signaling platforms.

KW - Bioimaging

KW - Bioorthogonal

KW - Fluorescent

UR - http://www.scopus.com/inward/record.url?scp=84976578095&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84976578095&partnerID=8YFLogxK

U2 - 10.1088/2050-6120/3/4/042001

DO - 10.1088/2050-6120/3/4/042001

M3 - Review article

AN - SCOPUS:84976578095

VL - 3

JO - Methods and Applications in Fluorescence

JF - Methods and Applications in Fluorescence

SN - 2050-6120

IS - 4

M1 - 042001

ER -