Biomonitoring of human genotoxicity induced by complex occupational exposures

B. Schoket, Miriam C. Poirier, Gábor Mayer, Géza Török, Ágnes Kolozsi-Ringelhann, Gabriella Bognár, William L. Bigbee, István Vincze

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Sensitivity, specificity and correlations among several biomarkers for monitoring occupational exposure to complex mixtures of genotoxic agents were assessed in occupational environments in Hungarian study populations. The studies have been focused on DNA adduct formation, urinary metabolites, mutations and micronuclei induced by exposures to complex organic mixtures. In two Hungarian aluminium plants, increased DNA adduct and 1-hydroxypyrene (1-OH-PY) levels were observed in workers as compared to controls. However, no association between the biomarker levels was evident on an individual basis. In Hungarian garage mechanics, DNA adduct determinations did not show increased genotoxic exposure as compared to the controls. However, ambient air measurements, significantly enhanced 1-OH-PY levels, and slightly enhanced frequency of micronuclei indicated increased polycyclic aromatic hydrocarbon (PAH) exposure in the garages, as compared to the general environment. In a Hungarian vulcanizing plant, DNA adduct determinations and 1-OH-PY did not show significantly elevated exposure levels as compared to controls. The glycophorin A (GPA) somatic mutation assay was also negative for this occupational exposure. The results support previous observations of a lack of correlation between DNA adducts detectable by 32P-postlabelling and those measured by the PAH-DNA immunoassay in the same DNA sample. These studies also demonstrate a lack of close correlation between levels of DNA adducts and urinary 1-OH-PY in the same individual.

Original languageEnglish
Pages (from-to)193-203
Number of pages11
JournalMutation Research - Genetic Toxicology and Environmental Mutagenesis
Volume445
Issue number2
DOIs
Publication statusPublished - Sep 30 1999

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DNA Adducts
Environmental Monitoring
Occupational Exposure
Polycyclic Aromatic Hydrocarbons
Complex Mixtures
Biomarkers
Plant DNA
Glycophorin
Mutation
DNA
Mechanics
Aluminum
Immunoassay
Air
Sensitivity and Specificity
hydroxide ion
Population

Keywords

  • 1-Hydroxypyrene
  • P-postlabelling
  • Aromatic DNA adduct
  • Biomarker
  • Competitive immunoassay
  • Glycophorin A
  • Human biomonitoring
  • Occupational exposure
  • Polycyclic aromatic hydrocarbon

ASJC Scopus subject areas

  • Health, Toxicology and Mutagenesis
  • Genetics

Cite this

Biomonitoring of human genotoxicity induced by complex occupational exposures. / Schoket, B.; Poirier, Miriam C.; Mayer, Gábor; Török, Géza; Kolozsi-Ringelhann, Ágnes; Bognár, Gabriella; Bigbee, William L.; Vincze, István.

In: Mutation Research - Genetic Toxicology and Environmental Mutagenesis, Vol. 445, No. 2, 30.09.1999, p. 193-203.

Research output: Contribution to journalArticle

Schoket, B, Poirier, MC, Mayer, G, Török, G, Kolozsi-Ringelhann, Á, Bognár, G, Bigbee, WL & Vincze, I 1999, 'Biomonitoring of human genotoxicity induced by complex occupational exposures', Mutation Research - Genetic Toxicology and Environmental Mutagenesis, vol. 445, no. 2, pp. 193-203. https://doi.org/10.1016/S1383-5718(99)00126-6
Schoket, B. ; Poirier, Miriam C. ; Mayer, Gábor ; Török, Géza ; Kolozsi-Ringelhann, Ágnes ; Bognár, Gabriella ; Bigbee, William L. ; Vincze, István. / Biomonitoring of human genotoxicity induced by complex occupational exposures. In: Mutation Research - Genetic Toxicology and Environmental Mutagenesis. 1999 ; Vol. 445, No. 2. pp. 193-203.
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