Biomimetic systems shed light on actin-based motility down to the molecular scale

Guillaume Romet-Lemonne, Emmanuel Helfer, Vincent Delatour, B. Bugyi, Montserrat Bosch, Stephane Romero, Marie France Carleir, Stephan Schmidt, Andreas Fery

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Cell motility, one of the modular activities of living cells, elicits the response of the cell to extra-cellular signals, to move directionally, feed, divide or transport materials. The combined actions of molecular motors and re-modeling of the cytoskeleton generate forces and movement. Here we describe mechanistic approaches of force and movement produced by site-directed assembly of actin filaments. The insight derived from a biochemical analysis of the protein machineries involved in "actin-based motile processes" like cell protrusions, invaginations, organelle propulsion, is used to build reconstituted assays that mimic cellular processes, using several protein machineries known to initiate filament assembly by different mechanisms. Reconstitution of complex self-organized systems presents a broad variety of interests. Reconstituting actin-based movement of a functionalized particle from a minimum number of pure proteins, first used to prove the general thermodynamic principles at work in motility, then was the basis for fully controlled physical measurements of forces produced by polymerization of actin against an obstacle and of the mechanical properties of the resulting polymer arrays. In addition, measurements at the mesoscopic scale (trajectories, velocity, polymer mechanics, fluorescence of specifically labeled components of the actin array, use of mutated proteins) can provide further insight into the molecular mechanisms underlying motility.

Original languageEnglish
Pages (from-to)5-15
Number of pages11
JournalBiophysical Reviews and Letters
Volume4
Issue number1-2
Publication statusPublished - 2009

Fingerprint

Biomimetics
Actins
Polymers
Proteins
Mechanics
Cytoskeleton
Actin Cytoskeleton
Thermodynamics
Polymerization
Organelles
Cell Movement
Fluorescence

Keywords

  • Actin-based motility
  • Biomimetism
  • Reconstituted systems

ASJC Scopus subject areas

  • Biophysics
  • Molecular Biology
  • Structural Biology

Cite this

Romet-Lemonne, G., Helfer, E., Delatour, V., Bugyi, B., Bosch, M., Romero, S., ... Fery, A. (2009). Biomimetic systems shed light on actin-based motility down to the molecular scale. Biophysical Reviews and Letters, 4(1-2), 5-15.

Biomimetic systems shed light on actin-based motility down to the molecular scale. / Romet-Lemonne, Guillaume; Helfer, Emmanuel; Delatour, Vincent; Bugyi, B.; Bosch, Montserrat; Romero, Stephane; Carleir, Marie France; Schmidt, Stephan; Fery, Andreas.

In: Biophysical Reviews and Letters, Vol. 4, No. 1-2, 2009, p. 5-15.

Research output: Contribution to journalArticle

Romet-Lemonne, G, Helfer, E, Delatour, V, Bugyi, B, Bosch, M, Romero, S, Carleir, MF, Schmidt, S & Fery, A 2009, 'Biomimetic systems shed light on actin-based motility down to the molecular scale', Biophysical Reviews and Letters, vol. 4, no. 1-2, pp. 5-15.
Romet-Lemonne G, Helfer E, Delatour V, Bugyi B, Bosch M, Romero S et al. Biomimetic systems shed light on actin-based motility down to the molecular scale. Biophysical Reviews and Letters. 2009;4(1-2):5-15.
Romet-Lemonne, Guillaume ; Helfer, Emmanuel ; Delatour, Vincent ; Bugyi, B. ; Bosch, Montserrat ; Romero, Stephane ; Carleir, Marie France ; Schmidt, Stephan ; Fery, Andreas. / Biomimetic systems shed light on actin-based motility down to the molecular scale. In: Biophysical Reviews and Letters. 2009 ; Vol. 4, No. 1-2. pp. 5-15.
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