Biomimetic Dextran-Based Hydrogel Layers for Cell Micropatterning over Large Areas Using the FluidFM BOT Technology

Andras Saftics, Barbara Türk, A. Sulyok, N. Nagy, Tamás Gerecsei, Inna Szekacs, Sándor Kurunczi, R. Horváth

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Micropatterning of living single cells and cell clusters over millimeter-centimeter scale areas is of high demand in the development of cell-based biosensors. Micropatterning methodologies require both a suitable biomimetic support and a printing technology. In this work, we present the micropatterning of living mammalian cells on carboxymethyl dextran (CMD) hydrogel layers using the FluidFM BOT technology. In contrast to the ultrathin (few nanometers thick in the dry state) CMD films generally used in label-free biosensor applications, we developed CMD layers with thicknesses of several tens of nanometers in order to provide support for the controlled adhesion of living cells. The fabrication method and detailed characterization of the CMD layers are also described. The antifouling ability of the CMD surfaces is demonstrated by in situ optical waveguide lightmode spectroscopy measurements using serum modeling proteins with different electrostatic properties and molecular weights. Cell micropatterning on the CMD surface was obtained by printing cell adhesion mediating cRGDfK peptide molecules (cyclo(Arg-Gly-Asp-d-Phe-Lys)) directly from aqueous solution using microchanneled cantilevers with subsequent incubation of the printed surfaces in the living cell culture. Uniquely, we present cell patterns with different geometries (spot, line, and grid arrays) covering both micrometer and millimeter-centimeter scale areas. The adhered patterns were analyzed by phase contrast microscopy and the adhesion process on the patterns was real-time monitored by digital holographic microscopy, enabling to quantify the survival and migration of cells on the printed cRGDfK arrays.

Original languageEnglish
JournalLangmuir
DOIs
Publication statusPublished - Jan 1 2019

Fingerprint

dextrans
Dextran
Hydrogel
biomimetics
Biomimetics
Dextrans
Hydrogels
cells
adhesion
Cells
bioinstrumentation
Biosensors
printing
Printing
Microscopic examination
Adhesion
microscopy
antifouling
Cell adhesion
phase contrast

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

Cite this

Biomimetic Dextran-Based Hydrogel Layers for Cell Micropatterning over Large Areas Using the FluidFM BOT Technology. / Saftics, Andras; Türk, Barbara; Sulyok, A.; Nagy, N.; Gerecsei, Tamás; Szekacs, Inna; Kurunczi, Sándor; Horváth, R.

In: Langmuir, 01.01.2019.

Research output: Contribution to journalArticle

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