Biomedical applications of protein microarrays

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

There is rapid development in the field of protein microarray technology with the promise of important advancements in the near future. Protein microarrays have been reportedly successful in serum tumor marker profiling as well as in drug discovery and medicinal chemistry when the effect of small molecules in protein-protein interaction is studied. Some of the bottlenecks of the technology are protein instability, problems with immobilization and stabilization of proteins to the corresponding surface, as well as aspecific and/or not preferred interactions and the lack of protein amplification techniques to generate sufficient amounts of low abundance proteins. For the time being, the number of genes in RNA expression chips is significantly greater than the number of proteins available for microchip based analysis of gene expression at the protein level. The automation and standardization routinely used with nucleic acid microarrays is not yet available in their protein chip counterparts. One of the emerging applications of protein microchips is biomarker discovery via chromatographic surface-based protein array techniques, which is applicable to minute amounts of samples with excellent detection limits using mass spectrometry based interrogation. In this paper the advantages, technical limitations and main biomedical application of protein microarrays are reviewed.

Original languageEnglish
Pages (from-to)2806-2815
Number of pages10
JournalCurrent Medicinal Chemistry
Volume16
Issue number22
DOIs
Publication statusPublished - 2009

Fingerprint

Protein Array Analysis
Microarrays
Proteins
Microchip Analytical Procedures
Biomarkers
Technology
Pharmaceutical Chemistry
Automation
Drug Discovery
Tumor Biomarkers
Immobilization
Nucleic Acids
Limit of Detection
Mass Spectrometry
RNA
Gene Expression
Gene expression
Standardization
Genes
Mass spectrometry

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology

Cite this

Biomedical applications of protein microarrays. / Spisák, S.; Guttman, A.

In: Current Medicinal Chemistry, Vol. 16, No. 22, 2009, p. 2806-2815.

Research output: Contribution to journalArticle

@article{d8c421357254484ab68c2eea4d387d55,
title = "Biomedical applications of protein microarrays",
abstract = "There is rapid development in the field of protein microarray technology with the promise of important advancements in the near future. Protein microarrays have been reportedly successful in serum tumor marker profiling as well as in drug discovery and medicinal chemistry when the effect of small molecules in protein-protein interaction is studied. Some of the bottlenecks of the technology are protein instability, problems with immobilization and stabilization of proteins to the corresponding surface, as well as aspecific and/or not preferred interactions and the lack of protein amplification techniques to generate sufficient amounts of low abundance proteins. For the time being, the number of genes in RNA expression chips is significantly greater than the number of proteins available for microchip based analysis of gene expression at the protein level. The automation and standardization routinely used with nucleic acid microarrays is not yet available in their protein chip counterparts. One of the emerging applications of protein microchips is biomarker discovery via chromatographic surface-based protein array techniques, which is applicable to minute amounts of samples with excellent detection limits using mass spectrometry based interrogation. In this paper the advantages, technical limitations and main biomedical application of protein microarrays are reviewed.",
author = "S. Spis{\'a}k and A. Guttman",
year = "2009",
doi = "10.2174/092986709788803141",
language = "English",
volume = "16",
pages = "2806--2815",
journal = "Current Medicinal Chemistry",
issn = "0929-8673",
publisher = "Bentham Science Publishers B.V.",
number = "22",

}

TY - JOUR

T1 - Biomedical applications of protein microarrays

AU - Spisák, S.

AU - Guttman, A.

PY - 2009

Y1 - 2009

N2 - There is rapid development in the field of protein microarray technology with the promise of important advancements in the near future. Protein microarrays have been reportedly successful in serum tumor marker profiling as well as in drug discovery and medicinal chemistry when the effect of small molecules in protein-protein interaction is studied. Some of the bottlenecks of the technology are protein instability, problems with immobilization and stabilization of proteins to the corresponding surface, as well as aspecific and/or not preferred interactions and the lack of protein amplification techniques to generate sufficient amounts of low abundance proteins. For the time being, the number of genes in RNA expression chips is significantly greater than the number of proteins available for microchip based analysis of gene expression at the protein level. The automation and standardization routinely used with nucleic acid microarrays is not yet available in their protein chip counterparts. One of the emerging applications of protein microchips is biomarker discovery via chromatographic surface-based protein array techniques, which is applicable to minute amounts of samples with excellent detection limits using mass spectrometry based interrogation. In this paper the advantages, technical limitations and main biomedical application of protein microarrays are reviewed.

AB - There is rapid development in the field of protein microarray technology with the promise of important advancements in the near future. Protein microarrays have been reportedly successful in serum tumor marker profiling as well as in drug discovery and medicinal chemistry when the effect of small molecules in protein-protein interaction is studied. Some of the bottlenecks of the technology are protein instability, problems with immobilization and stabilization of proteins to the corresponding surface, as well as aspecific and/or not preferred interactions and the lack of protein amplification techniques to generate sufficient amounts of low abundance proteins. For the time being, the number of genes in RNA expression chips is significantly greater than the number of proteins available for microchip based analysis of gene expression at the protein level. The automation and standardization routinely used with nucleic acid microarrays is not yet available in their protein chip counterparts. One of the emerging applications of protein microchips is biomarker discovery via chromatographic surface-based protein array techniques, which is applicable to minute amounts of samples with excellent detection limits using mass spectrometry based interrogation. In this paper the advantages, technical limitations and main biomedical application of protein microarrays are reviewed.

UR - http://www.scopus.com/inward/record.url?scp=70349389516&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70349389516&partnerID=8YFLogxK

U2 - 10.2174/092986709788803141

DO - 10.2174/092986709788803141

M3 - Article

C2 - 19689264

AN - SCOPUS:70349389516

VL - 16

SP - 2806

EP - 2815

JO - Current Medicinal Chemistry

JF - Current Medicinal Chemistry

SN - 0929-8673

IS - 22

ER -