Bioluminescent Monitoring of ATP Release from Human Red Blood Cells Treated with Nonionic Detergent

Tamäs Köszegi, Miklos Kellermayer, Ferenc Kövecs, Käzmer Jobst

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The adaptation of the firefly luciferin-luciferase system for the detection of ATP in the presence of Triton X 100 or Brij 58 nonionic detergents is described. The method is sensitive, easy to perform and is suitable for the continuous monitoring of the mobilization rate of ATP from Triton X 100- or Brij 58-treated human erythrocytes. A very rapid ATP release was observed after Triton X 100 treatment of the red blood cells while the presence of Brij 58 caused a retarded mobilization of the intracellular ATP. The technique described seems to be applicable to all kinds of cells.

Original languageEnglish
Pages (from-to)599-604
Number of pages6
JournalClinical Chemistry and Laboratory Medicine
Volume26
Issue number10
DOIs
Publication statusPublished - Jan 1 1988

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

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