Biologically active cholecystokinin octapeptide in rat small intestine

A. Varró, F. Hajnal

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Cholecystokinin is known to exist in several molecular forms; in the intestinal tract, besides CCK8, larger molecular forms are also present in considerable amounts. It was therefore surprising that in one study no bioactivity was found corresponding to CCK8 after Sephadex G50 chromatography of rat intestinal extract. This result was interpreted to mean that CCK8 in the rat intestine is not sulphated. We verified that non-sulphated CCK8 occurs in the intestinal tissue in negligible quantities; the compliance of the values of HPLC purified rat intestinal CCK8 sulphate (CCK8s) measured by bioassay and RIA (ratio:0.89 ± 0.01) proves unequivocally the presence of the bioactive CCK8s in the extract. A single major peak eluting in the position of synthetic CCK8 was observed when the HPLC purified CCK8s fraction from rat intestinal extract was rechromatographied by gel filtration of Sephadex G25, which supports the HPLC separation finding. Our data call attention to the necessity of careful handling of biological samples to avoid molecular damage during extraction and purification procedures.

Original languageEnglish
Pages (from-to)795
Number of pages1
JournalIRCS Medical Science
Volume11
Issue number9
Publication statusPublished - 1983

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Sincalide
Small Intestine
Rats
High Pressure Liquid Chromatography
Bioassay
Cholecystokinin
Chromatography
Bioactivity
Biological Assay
Sulfates
Compliance
Intestines
Purification
Gel Chromatography
Tissue
sephadex

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Biologically active cholecystokinin octapeptide in rat small intestine. / Varró, A.; Hajnal, F.

In: IRCS Medical Science, Vol. 11, No. 9, 1983, p. 795.

Research output: Contribution to journalArticle

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