Biological mechanisms underlying RU 486 clinical effects: Inhibition of endometrial stromal cell tissue factor content

Charles J. Lockwood, Graciela Krikun, C. Papp, Stefan Aigner, Yale Nemerson, Frederick Schatz

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Despite the pronounced hemorrhagic effects of RU 486 administration on luteal phase and early gestational endometrium, no information is available on the effect of RU 486 on endometrial hemostatic potential. The expression of endometrial stromal cell tissue factor (TF), the primary initiator of hemostasis, has been shown to be progestationally regulated in vivo and in vitro. To evaluate the effects of RU 486 on progestin-enhanced TF expression, confluent stromal cell cultures derived from proliferative phase endometria were exposed to vehicle control, 10-8 mol/L estradiol (E2), 10-6 mol/L dexamethasone, 10-7 mol/L medroxyprogesterone acetate (MPA), E2 plus MPA, E2 plus 10-6 mol/L progesterone (P), or 10-6 mol/L RU 486 alone or with E2 plus MPA or E2 plus P for 3-4 days. Compared to the vehicle control, E2, dexamethasone, and RU 486 alone had no effect on the content of immunoreactive and functionally active TF protein, whereas MPA increased and the combination of E2 and MPA further increased TF protein content. Similarly, E2 and P enhanced the stromal cell TF content. These progestin effects were blocked by RU 486. Similar results were obtained for steady state TF messenger ribonucleic acid (mRNA) levels. Possible RU 486-mediated reversal of progestin-enhanced stromal cell TF expression was assessed by incubating confluent cultures in E2 plus MPA for 3-10 days to enhance TF content, then washing the cultures and reexposing them to either E2 plus MPA or to RU 486 alone or with E2 plus MPA for 3, 4, or 7 days. Exposure to RU 486 alone or with E2 plus MPA greatly reduced levels of stromal cell TF protein and mRNA expression compared to those in cultures maintained in E2 plus MPA. These findings demonstrate that RU 486 not only blocks but also reverses in vitro progestin-enhanced stromal cell TF protein and mRNA expression, suggesting an additional mechanism for RU 486-induced menses and early abortion.

Original languageEnglish
Pages (from-to)786-790
Number of pages5
JournalJournal of Clinical Endocrinology and Metabolism
Volume79
Issue number3
DOIs
Publication statusPublished - Sep 1994

Fingerprint

Mifepristone
Medroxyprogesterone Acetate
Thromboplastin
Stromal Cells
Cells
Progestins
RNA
Endometrium
Dexamethasone
Proteins
Menstruation
Luteal Phase
Hemostatics
Hemostasis
Cell culture
Washing
Progesterone
Estradiol
Cell Culture Techniques

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Biological mechanisms underlying RU 486 clinical effects : Inhibition of endometrial stromal cell tissue factor content. / Lockwood, Charles J.; Krikun, Graciela; Papp, C.; Aigner, Stefan; Nemerson, Yale; Schatz, Frederick.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 79, No. 3, 09.1994, p. 786-790.

Research output: Contribution to journalArticle

Lockwood, Charles J. ; Krikun, Graciela ; Papp, C. ; Aigner, Stefan ; Nemerson, Yale ; Schatz, Frederick. / Biological mechanisms underlying RU 486 clinical effects : Inhibition of endometrial stromal cell tissue factor content. In: Journal of Clinical Endocrinology and Metabolism. 1994 ; Vol. 79, No. 3. pp. 786-790.
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