Biological evaluation of microbial toxin degradation by microinjected zebrafish (Danio rerio) embryos

Zsolt Csenki, Edina Garai, Anita Risa, Mátyás Cserháti, Katalin Bakos, Dalma Márton, Zoltán Bokor, Balázs Kriszt, B. Urbányi

Research output: Contribution to journalArticle

Abstract

The use of microinjection of newly fertilized zebrafish eggs as an appropriate tool for qualifying the biodetoxification properties of toxin-degrading microbes was investigated. Ochratoxin A (OTA), bacterial degradation products of OTA and bacterial metabolites of the Cupriavidus basilensis ŐR16 strain were microinjected. Results showed that variations in the injected droplet size, and thus treatment concentrations, stayed within ±20%, moreover embryo mortality did not exceed 10% in controls, that is in accordance with the recommendations of the OECD 236 guideline. The highest lethality was caused by OTA with a significantly higher toxicity than that of bacterial metabolites or OTA degradation products. However, toxicity of the latter two did not differ statistically from each other showing that the observed mortality was due to the intrinsic toxicity of bacterial metabolites (and not OTA degradation products), thus, the strain effectively degrades OTA to nontoxic products. Sublethal symptoms also confirmed this finding. Results: confirmed that microinjection of zebrafish embryos could be a reliable tool for testing the toxin-degrading properties of microbes. The method also allows comparisons among microbial strains able to degrade the same toxin, helping the selection of effective and environmentally safe microbial strains for the biodetoxification of mycotoxins in large scale.

Original languageEnglish
Pages (from-to)151-161
Number of pages11
JournalChemosphere
Volume227
DOIs
Publication statusPublished - Jul 1 2019

Fingerprint

Zebrafish
toxin
embryo
metabolite
Embryonic Structures
Metabolites
toxicity
Toxicity
Degradation
degradation
mortality
Microinjections
OECD
droplet
Cupriavidus
egg
Mortality
Mycotoxins
Zygote
evaluation

Keywords

  • Biodegradation
  • Biodetoxification
  • Cupriavidus basilensis
  • Mycotoxin
  • Ochratoxin

ASJC Scopus subject areas

  • Environmental Engineering
  • Environmental Chemistry
  • Chemistry(all)
  • Pollution
  • Health, Toxicology and Mutagenesis

Cite this

Biological evaluation of microbial toxin degradation by microinjected zebrafish (Danio rerio) embryos. / Csenki, Zsolt; Garai, Edina; Risa, Anita; Cserháti, Mátyás; Bakos, Katalin; Márton, Dalma; Bokor, Zoltán; Kriszt, Balázs; Urbányi, B.

In: Chemosphere, Vol. 227, 01.07.2019, p. 151-161.

Research output: Contribution to journalArticle

Csenki, Z, Garai, E, Risa, A, Cserháti, M, Bakos, K, Márton, D, Bokor, Z, Kriszt, B & Urbányi, B 2019, 'Biological evaluation of microbial toxin degradation by microinjected zebrafish (Danio rerio) embryos', Chemosphere, vol. 227, pp. 151-161. https://doi.org/10.1016/j.chemosphere.2019.04.014
Csenki, Zsolt ; Garai, Edina ; Risa, Anita ; Cserháti, Mátyás ; Bakos, Katalin ; Márton, Dalma ; Bokor, Zoltán ; Kriszt, Balázs ; Urbányi, B. / Biological evaluation of microbial toxin degradation by microinjected zebrafish (Danio rerio) embryos. In: Chemosphere. 2019 ; Vol. 227. pp. 151-161.
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