Biological and mechanical properties of platelet-rich fibrin membranes after thermal manipulation and preparation in a single-syringe closed system

Dorottya Kardos, István Hornyák, Melinda Simon, Adél Hinsenkamp, Bence Marschall, Róbert Várdai, Alfréd Kállay-Menyhárd, Balázs Pinke, László Mészáros, Olga Kuten, Stefan Nehrer, Zsombor Lacza

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Platelet-rich fibrin (PRF) membrane is a three-dimensional biodegradable biopolymer, which consists of platelet derived growth factors enhancing cell adhesion and proliferation. It is widely used in soft and hard tissue regeneration, however, there are unresolved problems with its clinical application. Its preparation needs open handling of the membranes, it degrades easily, and it has a low tensile strength which does not hold a suture blocking wider clinical applications of PRF. Our aim was to produce a sterile, suturable, reproducible PRF membrane suitable for surgical intervention. We compared the biological and mechanical properties of PRF membranes created by the classical glass-tube and those that were created in a single-syringe closed system (hypACT Inject), which allowed aseptic preparation. HypACT Inject device produces a PRF membrane with better handling characteristics without compromising biological properties. Freeze-thawing resulted in significantly higher tensile strength and higher cell adhesion at a lower degradation rate of the membranes. Mesenchymal stem cells seeded onto PRF membranes readily proliferated on the surface of fresh, but even better on freeze/thawed or freeze-dried membranes. These data show that PRF membranes can be made sterile, more uniform and significantly stronger which makes it possible to use them as suturable surgical membranes.

Original languageEnglish
Article number3433
JournalInternational journal of molecular sciences
Volume19
Issue number11
DOIs
Publication statusPublished - Nov 1 2018

Keywords

  • Biodegradation
  • Biopolymer
  • Medical device
  • Platelet-rich fibrin
  • Scaffold
  • Tissue regeneration

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Fingerprint Dive into the research topics of 'Biological and mechanical properties of platelet-rich fibrin membranes after thermal manipulation and preparation in a single-syringe closed system'. Together they form a unique fingerprint.

  • Cite this

    Kardos, D., Hornyák, I., Simon, M., Hinsenkamp, A., Marschall, B., Várdai, R., Kállay-Menyhárd, A., Pinke, B., Mészáros, L., Kuten, O., Nehrer, S., & Lacza, Z. (2018). Biological and mechanical properties of platelet-rich fibrin membranes after thermal manipulation and preparation in a single-syringe closed system. International journal of molecular sciences, 19(11), [3433]. https://doi.org/10.3390/ijms19113433