Biokinetic analysis of ubiquitin C-terminal hydrolase-L1 (UCH-L1) in severe traumatic brain injury patient biofluids

Gretchen M. Brophy, Stefania Mondello, Linda Papa, Steven A. Robicsek, Andrea Gabrielli, Joseph Tepas, A. Büki, Claudia Robertson, Frank C. Tortella, Ronald L. Hayes, Kevin K W Wang

Research output: Contribution to journalArticle

116 Citations (Scopus)

Abstract

Ubiquitin C-terminal hydrolase-L1 (UCH-L1) is a neuron-specific enzyme that has been identified as a potential biomarker of traumatic brain injury (TBI). The study objectives were to determine UCH-L1 exposure and kinetic metrics, determine correlations between biofluids, and assess outcome correlations in severe TBI patients. Data were analyzed from a prospective, multicenter study of severe TBI (Glasgow Coma Scale [GCS] score =≤8). Cerebrospinal fluid (CSF) and serum data from samples taken every 6 h after injury were analyzed by enzyme-linked immunosorbent assay (ELISA). UCH-L1 CSF and serum data from 59 patients were used to determine biofluid correlations. Serum samples from 86 patients and CSF from 59 patients were used to determine outcome correlations. Exposure and kinetic metrics were evaluated acutely and up to 7 days post-injury and compared to mortality at 3 months. There were significant correlations between UCH-L1 CSF and serum median concentrations (rs=0.59, ps=0.3, p=0.027), Tmax (r s=0.68, ps=0.65, p(0-24h) in those who died. This study shows a significant correlation between UCH-L1 CSF and serum median concentrations and biokinetics in severe TBI patients, and relationships with clinical outcome were detected.

Original languageEnglish
Pages (from-to)861-870
Number of pages10
JournalJournal of Neurotrauma
Volume28
Issue number6
DOIs
Publication statusPublished - Jun 1 2011

Fingerprint

Ubiquitin Thiolesterase
Cerebrospinal Fluid
Serum
Glasgow Coma Scale
Wounds and Injuries
Multicenter Studies
Biomarkers
Enzyme-Linked Immunosorbent Assay
Traumatic Brain Injury
Prospective Studies
Neurons
Mortality
Enzymes

Keywords

  • biomarkers
  • clinical trial
  • neural injury
  • outcome measures
  • traumatic brain injury

ASJC Scopus subject areas

  • Clinical Neurology

Cite this

Brophy, G. M., Mondello, S., Papa, L., Robicsek, S. A., Gabrielli, A., Tepas, J., ... Wang, K. K. W. (2011). Biokinetic analysis of ubiquitin C-terminal hydrolase-L1 (UCH-L1) in severe traumatic brain injury patient biofluids. Journal of Neurotrauma, 28(6), 861-870. https://doi.org/10.1089/neu.2010.1564

Biokinetic analysis of ubiquitin C-terminal hydrolase-L1 (UCH-L1) in severe traumatic brain injury patient biofluids. / Brophy, Gretchen M.; Mondello, Stefania; Papa, Linda; Robicsek, Steven A.; Gabrielli, Andrea; Tepas, Joseph; Büki, A.; Robertson, Claudia; Tortella, Frank C.; Hayes, Ronald L.; Wang, Kevin K W.

In: Journal of Neurotrauma, Vol. 28, No. 6, 01.06.2011, p. 861-870.

Research output: Contribution to journalArticle

Brophy, GM, Mondello, S, Papa, L, Robicsek, SA, Gabrielli, A, Tepas, J, Büki, A, Robertson, C, Tortella, FC, Hayes, RL & Wang, KKW 2011, 'Biokinetic analysis of ubiquitin C-terminal hydrolase-L1 (UCH-L1) in severe traumatic brain injury patient biofluids', Journal of Neurotrauma, vol. 28, no. 6, pp. 861-870. https://doi.org/10.1089/neu.2010.1564
Brophy, Gretchen M. ; Mondello, Stefania ; Papa, Linda ; Robicsek, Steven A. ; Gabrielli, Andrea ; Tepas, Joseph ; Büki, A. ; Robertson, Claudia ; Tortella, Frank C. ; Hayes, Ronald L. ; Wang, Kevin K W. / Biokinetic analysis of ubiquitin C-terminal hydrolase-L1 (UCH-L1) in severe traumatic brain injury patient biofluids. In: Journal of Neurotrauma. 2011 ; Vol. 28, No. 6. pp. 861-870.
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