Bioimprinted lipases in PVA nanofibers as efficient immobilized biocatalysts

Diána Weiser, Péter L. Sóti, Gergely Bánóczi, Viktória Bódai, Bálint Kiss, Ákos Gellért, Zsombor K. Nagy, Béla Koczka, A. Szilágyi, G. Marosi, L. Poppe

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15 Citations (Scopus)


Immobilization of lipases from Pseudomonas fluorescens (Lipase AK), Burkholderia (Pseudomonas) cepacia (Lipase PS) and lipase B from Pseudozyma (Candida) antarctica (CaLB) was investigated by entrapment in electrospun poly(vinyl alcohol) (PVA) nanofibers. The activity and selectivity of the lipases entrapped in PVA nanofibers were characterized in kinetic resolution of racemic secondary alcohols using acylation in organic media. Potential bioimprinting effect of eight substrate-mimicking additives [polyethylene glycols (PEGs), non-ionic detergents (NIDs) and various organosilanes] was tested with the fiber-entrapped lipases. The nanofibrous lipase biocatalyst entrapped in the presence of the additives were also characterized by rheology, differential scanning calorimetry and scanning electron microscopy. In addition to the known lipase-bioimprinting agents (PEGs, NIDs), phenyl- and octyltriethoxysilane also enhanced substantially the biocatalytic properties of lipases in their electrospun PVA fiber-entrapped forms. The reasons of bioimprinting effect of several additives were rationalized by docking studies in the open and closed form of CaLB.

Original languageEnglish
Publication statusAccepted/In press - Nov 18 2015



  • Electrospinning
  • Immobilization
  • Kinetic resolution
  • Lipase
  • Molecular imprinting
  • PVA nanofiber

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry
  • Drug Discovery

Cite this

Weiser, D., Sóti, P. L., Bánóczi, G., Bódai, V., Kiss, B., Gellért, Á., Nagy, Z. K., Koczka, B., Szilágyi, A., Marosi, G., & Poppe, L. (Accepted/In press). Bioimprinted lipases in PVA nanofibers as efficient immobilized biocatalysts. Tetrahedron.