Biofilm forming bacteria and archaea in thermal karst springs of Gellért Hill discharge area (Hungary)

A. Borsodi, Dóra Anda, Judit Makk, Gergely Krett, Péter Dobosy, Gabriella Büki, Anita Erőss, Judit Mádl-Szőnyi

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The Buda Thermal Karst System (BTKS) is an extensive active hypogenic cave system located beneath the residential area of the Hungarian capital. At the river Danube, several thermal springs discharge forming spring caves. To reveal and compare the morphological structure and prokaryotic diversity of reddish-brown biofilms developed on the carbonate rock surfaces of the springs, scanning electron microscopy (SEM), and molecular cloning were applied. Microbial networks formed by filamentous bacteria and other cells with mineral crystals embedded in extracellular polymeric substances were observed in the SEM images. Biofilms were dominated by prokaryotes belonging to phyla Proteobacteria, Chloroflexi and Nitrospirae (Bacteria) and Thaumarchaeota (Archaea) but their abundance showed differences according to the type of the host rock, geographic distance, and different water exchange. In addition, representatives of phyla Acidobacteria, Actinobacteria, Caldithrix, Cyanobacteria, Firmicutes Gemmatimonadetes, and several candidate divisions of Bacteria as well as Crenarchaeota and Euryarchaeota were detected in sample-dependent higher abundance. The results indicate that thermophilic, anaerobic sulfur-, sulfate-, nitrate-, and iron(III)-reducing chemoorganotrophic as well as sulfur-, ammonia-, and nitrite-oxidizing chemolithotrophic prokaryotes can interact in the studied biofilms adapted to the unique and extreme circumstances (e.g., aphotic and nearly anoxic conditions, oligotrophy, and radionuclide accumulation) in the thermal karst springs.

Original languageEnglish
JournalJournal of Basic Microbiology
DOIs
Publication statusAccepted/In press - Jan 1 2018

Fingerprint

Hungary
Archaea
Biofilms
Hot Temperature
Bacteria
Sulfur
Electron Scanning Microscopy
Crenarchaeota
Acidobacteria
Euryarchaeota
Chloroflexi
Proteobacteria
Actinobacteria
Carbonates
Cyanobacteria
Molecular Cloning
Nitrites
Ammonia
Rivers
Radioisotopes

Keywords

  • 16S rRNA gene diversity
  • archaea
  • bacteria
  • biofilm
  • Buda Thermal Karst System

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

Cite this

Biofilm forming bacteria and archaea in thermal karst springs of Gellért Hill discharge area (Hungary). / Borsodi, A.; Anda, Dóra; Makk, Judit; Krett, Gergely; Dobosy, Péter; Büki, Gabriella; Erőss, Anita; Mádl-Szőnyi, Judit.

In: Journal of Basic Microbiology, 01.01.2018.

Research output: Contribution to journalArticle

Borsodi, A. ; Anda, Dóra ; Makk, Judit ; Krett, Gergely ; Dobosy, Péter ; Büki, Gabriella ; Erőss, Anita ; Mádl-Szőnyi, Judit. / Biofilm forming bacteria and archaea in thermal karst springs of Gellért Hill discharge area (Hungary). In: Journal of Basic Microbiology. 2018.
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