Biofilm Bacterial Communities Inhabiting the Cave Walls of the Buda Thermal Karst System, Hungary

A. Borsodi, Mónika Knáb, Gergely Krett, Judit Makk, K. Márialigeti, Anita Eross, Judit Mádl-Szonyi

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

The diversity of biofilm bacterial communities associated with cave walls of the Buda Thermal Karst System (BTKS) located in Hungary was studied by scanning electron microscopy and molecular cloning based on 16S rRNA genes. Samples from two sites, the Molnár János cave (MJB) and the Rudas-Török spring cave (RTB), respectively, were analyzed and compared. The presence of iron precipitates was typical at both study sites, despite the fact that the cell morphological structure of the biofilms observed by SEM was characteristically different. Clones analyzed from BTKS were found to belong to 10 common phyla (Thermodesulfobacteria, Chloroflexi, Nitrospirae, Chlorobi, Proteobacteria, Firmicutes, Actinobacteria, Planctomycetes, Bacteroidetes, Verrucomicrobia) within the domain Bacteria. Moreover, sequences related to Aquificeae, Acidobacteria and Gemmatimonadetes were exclusive to MJB, while Cyanobacteria were found in RTB only. The phylogenetic distribution of the dominant bacterial clones was quite dissimilar between the two sites. In the biofilm from MJB clones affiliated with Firmicutes, whereas in the RTB clones related to Deltaproteobacteria were found in the highest number. In addition, substantially larger numbers of clone sequences related to thermophilic bacteria were recovered from MJB. On the basis of sequences of known microorganisms corresponding to our clone sequences, it is assumed that aerobic as well as anaerobic iron and sulfur transformation performed by different bacterial communities might be important biogenic processes in both caves.

Original languageEnglish
Pages (from-to)611-627
Number of pages17
JournalGeomicrobiology Journal
Volume29
Issue number7
DOIs
Publication statusPublished - Sep 2012

Fingerprint

Caves
Hungary
Biofilms
biofilm
cave
karst
clone
Clone Cells
Hot Temperature
Bacteria
Iron
Scanning electron microscopy
Cloning
Verrucomicrobia
Acidobacteria
Chlorobi
Deltaproteobacteria
scanning electron microscopy
Chloroflexi
Sulfur

Keywords

  • 16S rDNA
  • bacterial diversity
  • biofilm
  • clone library
  • karst cave
  • SEM

ASJC Scopus subject areas

  • Earth and Planetary Sciences (miscellaneous)
  • Microbiology
  • Environmental Science(all)
  • Environmental Chemistry

Cite this

Biofilm Bacterial Communities Inhabiting the Cave Walls of the Buda Thermal Karst System, Hungary. / Borsodi, A.; Knáb, Mónika; Krett, Gergely; Makk, Judit; Márialigeti, K.; Eross, Anita; Mádl-Szonyi, Judit.

In: Geomicrobiology Journal, Vol. 29, No. 7, 09.2012, p. 611-627.

Research output: Contribution to journalArticle

Borsodi, A. ; Knáb, Mónika ; Krett, Gergely ; Makk, Judit ; Márialigeti, K. ; Eross, Anita ; Mádl-Szonyi, Judit. / Biofilm Bacterial Communities Inhabiting the Cave Walls of the Buda Thermal Karst System, Hungary. In: Geomicrobiology Journal. 2012 ; Vol. 29, No. 7. pp. 611-627.
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