Biodistribution and function of extracellular miRNA-155 in mice

Shashi Bala, Timea Csak, Fatemeh Momen-Heravi, Dora Lippai, Karen Kodys, Donna Catalano, Abhishek Satishchandran, Victor Ambros, G. Szabó

Research output: Contribution to journalArticle

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Abstract

Circulating miRNAs can be found in extracellular vesicles (EV) and could be involved in intercellular communication. Here, we report the biodistribution of EV associated miR-155 using miR-155 KO mouse model. Administration of exosomes loaded with synthetic miR-155 mimic into miR-155 KO mice resulted in a rapid accumulation and clearance of miR-155 in the plasma with subsequent distribution in the liver, adipose tissue, lung, muscle and kidney (highest to lowest, respectively). miR-155 expression was detected in isolated hepatocytes and liver mononuclear cells of recipient KO mice suggesting its cellular uptake. In vitro, exosome-mediated restoration of miR-155 in Kupffer cells from miR-155 deficient mice augmented their LPS-induced MCP1 mRNA increase. The systemic delivery of wild type plasma to miR-155 KO mice also resulted in a rapid accumulation of miR-155 in the circulation and distribution to the liver and adipose tissue. In summary, our results demonstrate tissue biodistribution and biologic function of EV-associated miR-155.

Original languageEnglish
Article number10721
JournalScientific Reports
Volume5
DOIs
Publication statusPublished - May 29 2015

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MicroRNAs
Exosomes
Adipose Tissue
Liver
Kupffer Cells
Hepatocytes
Kidney
Muscles
Lung
Messenger RNA
Mouse Mirn155 microRNA
Extracellular Vesicles

ASJC Scopus subject areas

  • General

Cite this

Bala, S., Csak, T., Momen-Heravi, F., Lippai, D., Kodys, K., Catalano, D., ... Szabó, G. (2015). Biodistribution and function of extracellular miRNA-155 in mice. Scientific Reports, 5, [10721]. https://doi.org/10.1038/srep10721

Biodistribution and function of extracellular miRNA-155 in mice. / Bala, Shashi; Csak, Timea; Momen-Heravi, Fatemeh; Lippai, Dora; Kodys, Karen; Catalano, Donna; Satishchandran, Abhishek; Ambros, Victor; Szabó, G.

In: Scientific Reports, Vol. 5, 10721, 29.05.2015.

Research output: Contribution to journalArticle

Bala, S, Csak, T, Momen-Heravi, F, Lippai, D, Kodys, K, Catalano, D, Satishchandran, A, Ambros, V & Szabó, G 2015, 'Biodistribution and function of extracellular miRNA-155 in mice', Scientific Reports, vol. 5, 10721. https://doi.org/10.1038/srep10721
Bala S, Csak T, Momen-Heravi F, Lippai D, Kodys K, Catalano D et al. Biodistribution and function of extracellular miRNA-155 in mice. Scientific Reports. 2015 May 29;5. 10721. https://doi.org/10.1038/srep10721
Bala, Shashi ; Csak, Timea ; Momen-Heravi, Fatemeh ; Lippai, Dora ; Kodys, Karen ; Catalano, Donna ; Satishchandran, Abhishek ; Ambros, Victor ; Szabó, G. / Biodistribution and function of extracellular miRNA-155 in mice. In: Scientific Reports. 2015 ; Vol. 5.
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