Biodegradation of sulfanilic acid by Pseudomonas paucimobilis

K. Perei, G. Rákhely, I. Kiss, B. Polyák, K. Kovács

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

An aerobic bacterium, isolated from a contaminated site, was able to degrade sulfanilic acid (4-aminobenzenesulfonic acid) and was identified as Pseudomonas paucimobilis. The isolate could grow on sulfanilic acid (SA) as its sole carbon and nitrogen source and metabolized the target compound to biomass. The bioconversion capacity depended on the sulfanilic acid concentration; greater than 98% elimination of the hazardous compound was achieved at low (10 mM) sulfanilic acid concentration, and the yield was greater than 70% at 50 mM concentration of the contaminant. The maximum conversion rate was 1.5 mmol sulfanilic acid/h per mg wet cells at 30°C. Ca-alginate-phytagel proved a good matrix for immobilization of P. paucimobilis, with essentially unaltered biodegradation activity. Removal of sulfanilic acid from contaminated industrial waste water was demonstrated. SDS-PAGE analysis of the crude extract revealed novel proteins appearing upon induction with sulfanilic acid and related compounds, which indicated alternative degradation mechanisms involving various inducible enzymes.

Original languageEnglish
Pages (from-to)101-107
Number of pages7
JournalApplied Microbiology and Biotechnology
Volume55
Issue number1
DOIs
Publication statusPublished - 2001

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Sulfanilic Acids
Pseudomonas
Biodegradation
Acids
Aerobic bacteria
Industrial Waste
Bioconversion
Aerobic Bacteria
Complex Mixtures
Waste Water
Industrial wastes
Alginate
Immobilization
Biomass
Wastewater
Nitrogen
Carbon
Polyacrylamide Gel Electrophoresis
Impurities
Degradation

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology
  • Bioengineering

Cite this

Biodegradation of sulfanilic acid by Pseudomonas paucimobilis. / Perei, K.; Rákhely, G.; Kiss, I.; Polyák, B.; Kovács, K.

In: Applied Microbiology and Biotechnology, Vol. 55, No. 1, 2001, p. 101-107.

Research output: Contribution to journalArticle

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