Biochemical mapping of the noradrenergic projection from the locus coeruleus: A model for studies of brain neuronal pathways

Ronald M. Kobayashi, M. Palkóvits, David M. Jacobowitz, Irwin J. Kopin

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

Mapping of the noradrenergic projection from neurons in the rat locus coeruleus has been examined by combining a sensitive radioisotopic assay for catecholamines with a microdissection technique to remove multiple separate brain nuclei. The effect of a unilateral locus coeruleus lesion on norepinephrine concentration in 19 brain regions ipsilateral and contralateral to the lesion was determined. Evidence for ipsilateral and bilateral innervation to specific regions is presented, and many regions appear to receive combined innervation from other noradrenergic loci, in addition to that from the locus coeruleus. Fluorescence rating was correlated with biochemical measurement of amine content with these techniques and proportionality was observed over a narrow range. With this proportionality taken into consideration, mapping results obtained by biochemical and fluorescence methods are compared.

Original languageEnglish
Pages (from-to)223-233
Number of pages11
JournalNeurology
Volume25
Issue number3
Publication statusPublished - 1975

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Locus Coeruleus
Brain
Fluorescence
Adrenergic Neurons
Microdissection
Catecholamines
Amines
Norepinephrine
Locus
Pathway
Lesion
Proportionality

ASJC Scopus subject areas

  • Arts and Humanities (miscellaneous)
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Biochemical mapping of the noradrenergic projection from the locus coeruleus : A model for studies of brain neuronal pathways. / Kobayashi, Ronald M.; Palkóvits, M.; Jacobowitz, David M.; Kopin, Irwin J.

In: Neurology, Vol. 25, No. 3, 1975, p. 223-233.

Research output: Contribution to journalArticle

Kobayashi, Ronald M. ; Palkóvits, M. ; Jacobowitz, David M. ; Kopin, Irwin J. / Biochemical mapping of the noradrenergic projection from the locus coeruleus : A model for studies of brain neuronal pathways. In: Neurology. 1975 ; Vol. 25, No. 3. pp. 223-233.
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