Biochemical, Histopathological and Molecular Responses in Gills of Leuciscus cephalus Exposed to Metals

Anca Hermenean, Georgiana Gheorghiu, Miruna Silvia Stan, Hildegard Herman, Bianca Onita, Doru Puiu Ardelean, Aurel Ardelean, M. Braun, M. Zsuga, S. Kéki, Marieta Costache, Anca Dinischiotu

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Gills are major targets for acute metal toxicity in fish, due to their permanent contact with aquatic pollutants. To assess the effects of metals on gills of the Leuciscus cephalus (chub), fish individuals were collected from two sites in the Tur River, Romania, in upstream (site 1) and downstream (site 2) of a metal pollution source. Quantitative and hyperspectral analyses showed that Zn, Sr, and Fe concentrations were significantly higher in gills from site 2 compared with site 1. Malondialdehyde and advanced oxidation protein products levels increased 17 and 28%, respectively, whereas reduced glutathione level diminished significantly in the gills of fish collected from site 2 compared to site 1. The activities of superoxide dismutase, catalase, and glutathione-S-transferase increased significantly at 41, 21, and 28%, respectively. Proliferating cell nuclear antigen (PCNA) protein levels, as well as the amount of DNA damage, were significantly increased for site 2 compared with site 1. The induced oxidative stress generated hyperplasia, hypertrophy, and inflammation in the epithelial cells and apoptosis. Hence, this could suggest that gill cells have tried to counteract the oxidative stress-induced DNA fragmentation by PCNA up-regulation, but the PCNA expression decreased on longer time due to the low level of GSH, resulting in apoptosis.

Original languageEnglish
Pages (from-to)607-618
Number of pages12
JournalArchives of Environmental Contamination and Toxicology
Volume73
Issue number4
DOIs
Publication statusPublished - Nov 1 2017

Fingerprint

Proliferating Cell Nuclear Antigen
Antigens
Fish
Oxidative stress
Metals
Cell death
DNA
Advanced Oxidation Protein Products
Fishes
Apoptosis
Proteins
Glutathione Transferase
Malondialdehyde
Oxidative Stress
Catalase
Superoxide Dismutase
Glutathione
Toxicity
Pollution
Cyprinidae

ASJC Scopus subject areas

  • Toxicology
  • Pollution
  • Health, Toxicology and Mutagenesis

Cite this

Biochemical, Histopathological and Molecular Responses in Gills of Leuciscus cephalus Exposed to Metals. / Hermenean, Anca; Gheorghiu, Georgiana; Stan, Miruna Silvia; Herman, Hildegard; Onita, Bianca; Ardelean, Doru Puiu; Ardelean, Aurel; Braun, M.; Zsuga, M.; Kéki, S.; Costache, Marieta; Dinischiotu, Anca.

In: Archives of Environmental Contamination and Toxicology, Vol. 73, No. 4, 01.11.2017, p. 607-618.

Research output: Contribution to journalArticle

Hermenean, A, Gheorghiu, G, Stan, MS, Herman, H, Onita, B, Ardelean, DP, Ardelean, A, Braun, M, Zsuga, M, Kéki, S, Costache, M & Dinischiotu, A 2017, 'Biochemical, Histopathological and Molecular Responses in Gills of Leuciscus cephalus Exposed to Metals', Archives of Environmental Contamination and Toxicology, vol. 73, no. 4, pp. 607-618. https://doi.org/10.1007/s00244-017-0450-5
Hermenean, Anca ; Gheorghiu, Georgiana ; Stan, Miruna Silvia ; Herman, Hildegard ; Onita, Bianca ; Ardelean, Doru Puiu ; Ardelean, Aurel ; Braun, M. ; Zsuga, M. ; Kéki, S. ; Costache, Marieta ; Dinischiotu, Anca. / Biochemical, Histopathological and Molecular Responses in Gills of Leuciscus cephalus Exposed to Metals. In: Archives of Environmental Contamination and Toxicology. 2017 ; Vol. 73, No. 4. pp. 607-618.
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