Biochemical characterization of Siberian sturgeon Acipenser baeri and sterlet Acipenser ruthenus milt plasma and spermatozoa

B. Piros, J. Glogowski, R. Kolman, A. Rzemieniecki, J. Domagala, A. Horváth, B. Urbányi, A. Ciereszko

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

This paper provides data concerning biochemical composition of milt of two sturgeon species, Siberian sturgeon bred in aquaculture facility of Inland Fisheries Institute in North Poland and sterlet (from two different populations from Danube and Odra). Milt plasma of Siberian sturgeon and sterlet, when compared to teleost fish, is characterized by much lower osmolality (up 70 to 96 mOsm kg-1) and protein concentration (0.24-0.58 g l-1). In spite of the presence of an acrosome and acrosin the antiproteinase activity of seminal plasma was low (12.79-25.40 U l-1). Activities of arylsulfatase and β-N-acetylglucosaminidase were found in spermatozoa. This agrees with the presence of an acrosome in sturgeons sperm. Similarly to mammals, these enzymes are also present in milt plasma. We determined a range of enzymatic activities from the minimal (seminal plasma) to the maximal damage (supernatants obtained after freezing-thawing without cryoprotectant). Activities of arylsulfatase, β-N-acetylglucosaminidase, lactic dehydrogenase and acid phosphatase were released from spermatozoa after freezing-thawing. For this reason they are good potential candidates as a markers of cryoinjury to sperm acrosome and midpiece.

Original languageEnglish
Pages (from-to)289-295
Number of pages7
JournalFish Physiology and Biochemistry
Volume26
Issue number3
DOIs
Publication statusPublished - 2002

Fingerprint

milt
Acrosome
Arylsulfatases
Spermatozoa
Acetylglucosaminidase
acrosome
spermatozoa
Semen
Plasmas
plasma
Freezing
arylsulfatase
Thawing
freeze-thaw cycles
seminal plasma
Sperm Midpiece
sturgeon
thawing
Acrosin
sperm

Keywords

  • Milt
  • Seminal plasma
  • Siberian sturgeon
  • Spermatozoa
  • Sterlet

ASJC Scopus subject areas

  • Aquatic Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Physiology
  • Physiology (medical)

Cite this

Biochemical characterization of Siberian sturgeon Acipenser baeri and sterlet Acipenser ruthenus milt plasma and spermatozoa. / Piros, B.; Glogowski, J.; Kolman, R.; Rzemieniecki, A.; Domagala, J.; Horváth, A.; Urbányi, B.; Ciereszko, A.

In: Fish Physiology and Biochemistry, Vol. 26, No. 3, 2002, p. 289-295.

Research output: Contribution to journalArticle

Piros, B. ; Glogowski, J. ; Kolman, R. ; Rzemieniecki, A. ; Domagala, J. ; Horváth, A. ; Urbányi, B. ; Ciereszko, A. / Biochemical characterization of Siberian sturgeon Acipenser baeri and sterlet Acipenser ruthenus milt plasma and spermatozoa. In: Fish Physiology and Biochemistry. 2002 ; Vol. 26, No. 3. pp. 289-295.
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