Biochemical changes in tissue catecholamines and serotonin in duodenal ulceration caused by cysteamine or propionitrile in the rat

S. Szabo, H. C. Horner, H. Maull, J. Schnoor, C. C. Chiueh, M. Palkóvits

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Abstract

Previous structure-activity and pharmacologic studies with duodenal ulcerogens cysteamine and propionitrile implicating catecholamines in the pathogenesis of duodenal ulceration have now been followed up by dose- and time-response biochemical investigations to assess the importance of monoamines in the development of duodenal ulcers. The concentrations of norepinephrine (noradrenaline), dopamine, serotonin and their metabolites were measured in total brain, brain regions, stomach, duodenum, pancreas and adrenals in the rat. Turnover of catecholamines was determined in rats pretreated with the inhibitor of tyrosine hydroxylase α-methyl-p-tyrosine. The duodenal ulcerogens caused a dose- and time-dependent depletion of norepinephrine in virtually all the tissues examined. The effect was maximal 4 or 7 hr after cysteamine or propionitrile, and norepinephrine levels returned to normal in 24 hr. Dopamine changes were selective and often biphasic, e.g., elevation in adrenals, biphasic in brain cortex, hippocampus and midbrain, but uniformly decreasing in glandular stomach and duodenum. In the median eminence dopamine levels decreased by 181 and 324% at 15 and 30 min, respectively, after cysteamine, but neither dopamine nor 3,4-dihydroxyphenylacetic acid was modified in the periventricular nucleus. Serotonin levels were relatively stable, revealing slight elevations or no changes in most of the tissues. The turnover of norepinephrine was accelerated by both chemicals in virtually all brain regions, but dopamine turnover was affected only in a few areas, e.g., in the corpus striatum and medulla oblongata cysteamine decreased dopamine turnover, whereas propionitrile first (at 1 hr) accelerated then (at 8 hr) significantly suppressed it. Correlation of ulcer intensity after a single dose of cysteamine with concentrations of monoamines revealed a significant negative association between dopamine levels in the brain and duodenal ulcer severity (r = -0.633, P

Original languageEnglish
Pages (from-to)871-878
Number of pages8
JournalJournal of Pharmacology and Experimental Therapeutics
Volume240
Issue number3
Publication statusPublished - 1987

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Cysteamine
Catecholamines
Dopamine
Serotonin
Norepinephrine
Brain
Duodenal Ulcer
Duodenum
Stomach
Corpus Striatum
3,4-Dihydroxyphenylacetic Acid
Medulla Oblongata
Median Eminence
Tyrosine 3-Monooxygenase
Mesencephalon
propionitrile
Ulcer
Tyrosine
Pancreas
Hippocampus

ASJC Scopus subject areas

  • Pharmacology

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Biochemical changes in tissue catecholamines and serotonin in duodenal ulceration caused by cysteamine or propionitrile in the rat. / Szabo, S.; Horner, H. C.; Maull, H.; Schnoor, J.; Chiueh, C. C.; Palkóvits, M.

In: Journal of Pharmacology and Experimental Therapeutics, Vol. 240, No. 3, 1987, p. 871-878.

Research output: Contribution to journalArticle

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