Bioavailability- and bioaccessibility-dependent mutagenicity of pentachlorophenol (PCP)

Csilla Hajdu, Katalin Gruiz, Éva Fenyvesi

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7 Citations (Scopus)

Abstract

The biological effect of a soil pollutant depends on Its actual bioavailability and bioaccessibility, which is associated with the Kp value (soil-solid-soil-water partition coefficient) in the soil, and with the interaction between the soil and the test organism. Our aim is to find a biologically relevant conservative model for assessing the biologically effective proportion of the contaminant by increasing the bioavailability and/or bioaccessibility of the contaminant to a value that can be considered as the maximum in a biological system in the environment, for example, in freshwater or soil ecosystems. We selected cyclodextrin, a starch-type molecule, in order to develop a reproducible interactive bioassay based on a pessimistic approach, but still remaining within a biologically realistic range. Cyclodextrin (CD) can be applied in nanotechnology, which allows us to modify the matrix-effect of the soil by simulating the maximum bioavailability/bioaccessibility of organic contaminants through their inclusion using cyclodextrin. We introduce the mutagenicity testing of pentachlorophenol (PCP), a common, high-KQW (octanolwater partition coefficient) contaminant in soil with and without cyclodextrin pre-treatment. As an alternative to the cyclodextrin model, traditional bioavailability/bioaccessibility enhancement by simulating the process of human digestion was also tested and compared on contaminated soils. PCP has a high KQw and its mutagenicity is questionable, due to controversial data from the literature. After complexation using RAMEB (randomly methylated β-cyclodextrin), a significant mutagenic effect was measured in PCP-contaminated soil. The same soil was negative even after simulated human digestion

Original languageEnglish
Pages (from-to)473-481
Number of pages9
JournalLand Contamination and Reclamation
Volume17
Issue number3-4
DOIs
Publication statusPublished - Dec 1 2009

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Keywords

  • Ames test
  • Bioaccessibility
  • Bioavailability
  • Cyclodextrin
  • Ecotoxicology
  • Mutagenicity
  • Nanotechnology
  • Pentachlorophenol
  • RAMEB
  • Soil
  • Whole-soil test

ASJC Scopus subject areas

  • Water Science and Technology
  • Geotechnical Engineering and Engineering Geology
  • Environmental Science(all)

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